The Great Plains Laboratory has performed more than 100000 tests of persons on the autistic spectrum throughout the world Free consultation with testing by phoneSkype 2 autistic children of Great Plains employees have completely recovered using suggestions of Great Plains ID: 559476
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Slide1Slide2
Why use The Great Plains Laboratory?
The Great Plains Laboratory has performed more than 100,000 tests of persons on the autistic spectrum throughout the world.
Free consultation with testing by phone/Skype
2 autistic children of Great Plains employees have completely recovered using suggestions of Great Plains
Licensed by the USA government
Performs external proficiency testing for all substances for which it does testing, including many of which are voluntary
Great customer service by highly experienced staff.Slide3
How to obtain test kits
Contact The Great Plains Laboratory by phone, fax, or email
Phone 1 913 341-8949
Fax 1 913 341-6207
Email:
gpl4u@aol.com
Videos on how to prepare the test samples on the website at www.greatplainslaboratory.comSlide4
Test kits
Contain all necessary containers to transport samples
Contains information to ship by FEDEX
Contains all necessary information to ship samples
Contains payment information, MasterCard, Visa, or electronic bank transferSlide5
ORGANIC ACID
TEST-Great Plains
72
Compounds
and ratios
Yeast
/
Fungal Metabolites-no Bacterial Metabolites-no B-vitamin Deficiencies-no Antioxidant Deficiencies-no Inborn Errors of Metabolism High Oxalate Levels-no
Neurotransmitter
metabolism
-no
Indications
of
diabetic
conditions
Citric
acid
(
Krebs
)
cycle
metabolites
Fatty
acid
abnormalities
Clostridia
overgrowth
-no
Glycolysis
metabolites
Amino
Acid
metabolites
Pyrimidines
-no
72
compounds
totalSlide6
Specific autism markers present on The Great Plains Laboratory Organic Acid Test
Slide7
Normal intestine
=bad bacteria
Intestine
Y = yeast
* = yeast product
Blood vessel
Rest of body
Kidney
Urine
cup
Y *
B
Y
*
*
*
*
*
B
B
B
B
B
B
B
B
B
B
B
B
B B
B
B
B
B B
B
B
B
B
B
B B
B
B = good bacteriaSlide8
Y
Y
Y
Y
Y
Y
*Y
Y
Y**
*
*
*
*
*
*
*
*
*
*
*
*
*
****
*******
*
*****
*
*
*
*
*****
******
*
*
*
*****
*
*
**
*
*
*
*
*
*
*
* * * *
*
**
*
*
B
*
*
*
*
***
*
**
*
B
*****
*****
*****
**
**
**
**
****
****
****
****
*******
*****
*****
**
** *
* * *
* *
After antibiotics
=bad bacteria
Intestine
Y = yeast
* = yeast product
Blood vessel
Rest of body
Kidney
Urine
cup
B = good bacteriaSlide9
Nutritional Neuroscience 2010 Vol 13 No 3: 1-10Slide10
HPHPA
Shaw, W Increased Urinary Excretion of Analogs of Krebs Cycle Metabolites and Arabinose in Two Brothers with Autistic Features.
Clin
Chem
41:1094-1104, 1995Slide11
C. difficile-pseudomenbranous colitis
C. sporogenesC. botulinum-food poisoning
C. mangenoti
C. ghoni
C. bifermentans
C. caloritolerans
Clostridia species that produce HPHPA precursors
Arch Microbiol 1976; 107:283–288Slide12
Two months of
nystatin and Lactobacillus
acidophilus GG therapy in a child with autismSlide13
CH
2
CHCOOH
NH
2
CH
2
CHCOOH
NH
2
HO
tyrosine
CH
2
CHCOOH
NH
2
HO
DOPA
HO
Dopamine
Norepinephrine
phenylalanine
phenylalanine
hydroxylase
tyrosine
hydroxylase
CH
2
CH
2
NH
2
HO
HO
Dopamine
beta-hydroxylase
DOPA
decarboxylase
CHCH
2
NH
2
HO
HO
OH
X
CH
2
CH
2
NH
2
HO
HO
Clostridia dopamine
analog
HVA
VMA
3-hydroxyphenylalanine
Clostridia DOPA analogSlide14Slide15
HVA/VMA= 5:1
Dopamine
Norepi
,
Epi
Altered dopamine/norepinephrine ratio with Clostridia excess
Dopamine/
Norepi
, Epi Slide16
HVA/VMA= 2.58 (Excess dopamine)
Before treatment (depression)Slide17
After treatment
HVA/VMA= 1.38Slide18
J Child
Neurol
2000 Jul;15(7):429-35
Short-term benefit from oral vancomycin treatment of regressive-onset autism.
Sandler RH,
Finegold
SM,
Bolte
ER, Buchanan CP, Maxwell AP, Vaisanen ML, Nelson,MN, Wexler HM11 children with regressive-onset autism were recruited for an intervention trial using vancomycin. A clinical psychologist blinded to treatment status..noted improvement in 8 of 10 children studied. “…these results indicate that a possible gut flora-brain connection warrants further investigation” Slide19Slide20Slide21Slide22Slide23
Staghorn
oxalate crystal in kidneySlide24
Autistic
Spectrum
N=100
Normal
Children
N=16
p <10
-16
t-testComparison of urine oxalate in autistic spectrum and normal childrenSlide25
Children exposed to pesticides called organophosphates used to kill insects had more than twice the risk of developing pervasive developmental disorder (PDD)
For
organochlorines
, there was 7X autism rate
Mothers exposed to such pesticides were also likely to have shorter pregnancies and their children to have impaired reflexes
Maternal Residence Near Agricultural Pesticide Applications and Autism Spectrum Disorders among Children in the California Central Valley
Eric M. Roberts, et al. Environ Health
Perspect. 115(10): 1482–1489, 2007Slide26
I. Hertz-
Picciotto, et al Public Health Sciences and the M.I.N.D. Institute, University of California at Davis, International Meeting for Autism Research (IMFAR) Household Pesticide Use in Relation to Autism, May 2008
Mothers exposed to pet shampoos (almost all pyrethrins) were twice as likely to have a child with autism.
The worst exposure time was the second trimester.Slide27Slide28Slide29Slide30
R Cade et al.
Autism and schizophrenia: Intestinal disorders. Nutritional Neuroscience 3:
57-72,2000 Depts
. of Medicine, Physiology, Psychology, and Psychiatry, University of Florida, USA
Abnormal peptides in both patients with autism and schizophrenia
High titers of IgG antibodies to
gliadin
(
wheat) found in 87% of patients with autism and 86% of patients with schizophreniaHigh titers of IgG antibodies to bovine casein found in 90% of patients with autism and 93% of patients with schizophreniaA gluten and casein free diet caused significant improvement in 81% of patients with autism within 3 months Slide31
Efficacy of a cow's milk free diet (or other foods which gave a positive result after a skin test) in 36 autistic patients.
Marked improvement in the behavioral symptoms of patients after a period of 8 weeks on an elimination diet
High levels of
IgA
antigen specific antibodies for casein,
lactalbumin
and beta-
lactoglobulin
High IgG and IgM for casein Lucarelli S, Panminerva Med 1995 Sep;37(3):137-41 Food allergy and infantile autism.Slide32
normal
autism
Great Plains dataSlide33
IgG Comprehensive Food
Allergy Test - AutismSlide34
Blood draw
Fingerstick done home
Blood draw only
IgG antibody test
IgE antibody testSlide35Slide36Slide37
Great Plains Immune Deficiency ProfileSlide38
Great Plains Immune Deficiency ProfileSlide39
Great Plains Immune Deficiency ProfileSlide40
Immune deficiencies in autism
IgA deficiency
Total IgG deficiency
IgG subclass deficiency
Complement C4b deficiency
Myeloperoxidase deficiency
Severe combined immunodeficiency disorder (SCID)Normal antibody concentrations but lack of specific antibodies, even though multiple vaccines givenSlide41
Abnormally elevated
IgE in child with autism and severe self abuseSlide42
Autism and seizures
Slide43
Recurrence of Candida: The most difficult medical problem in autism
Because of depression of cellular immunity, the person with autism becomes re-infected immediately after antifungal treatment stops
Some parents report deterioration if even a single dose of antifungal drug is missed
Some children on antifungals for over 10 yearsSlide44
Streptococcus antibodies and
autism-contribute to stereotypical behaviorSlide45
Streptococcus antibodies and autism
followup
Throat rapid strep test
Throat culture
Cultures may give false negative results
Group A beta-hemolytic streptococcus
Antibodies may cause behavioral alteration even if strep organism is missing
Prophylactic antibiotics Plasmapharesis and replacementSelective removal of plasma anti-strep antibodiesIVIG-itravenous gamma globulinSlide46
Hair metals in boys with autism
n=40 boys per group, age matched
Autism 9:290-298,2005Slide47
Chelation
Therapy Options
“To Bind” from Greek word
chele
, or claw.
Oral DMSA or TD-DMSA TD=
transdermal
Oral DMPS and/or TD-DMPS Oral EDTA and/or TD-EDTAIntravenous – DMPS, EDTA
Suppository – Detoxamin (good for lead, aluminum)Natural Remedies – Chlorella, Cilantro, Homeopathic, etc.Sublingual vs Baths.Combination of different forms.Slide48
Recommended testing summary
Hair screen for long term exposure to toxic elements
If all toxic elements are negative with hair metals, consider urine challenge test with DMSA,DMPS
Evaluate copper/zinc balance with copper/zinc profile from Great Plains
Use urine test (without challenge) to evaluate calcium levels
Fecal metals can indicate oral exposure to metals especially lead but does not identify source
Use X-ray fluorescence to determine
sources of leadWhole blood or RBC good for acute toxicitySlide49
Recommended treatment
Find and eliminate sources of toxicity
Remove amalgams
Control Candida since DMSA and DMPS stimulate Candida overgrowth
Oral DMSA and DMPS cheap, safe, and effective but stimulate Candida and can make heavy metals worse if oral ingestion is still occurring.
Extended treatment needed to remove toxic metals from brain
Do periodic liver tests and blood counts
Supplement with beneficial metals (Chelate-Mate®)
Discontinue if tests abnormal or rare severe symptomsSlide50
Safety and Efficacy of Oral DMSA Therapy for Children with Autism Spectrum
Disorders: Part B - Behavioral ResultsBMC Clinical Pharmacology 2009, 9:17 doi:10.1186/1472-6904-9-17James B Adams (jim.adams@asu.edu)
Matthew
Baral
(m.baral@scnm.edu)
Elizabeth
Geis
(autismstudynurseasu@gmail.com)Jessica Mitchell (j.mitchell@scnm.edu)Julie Ingram (julieaingram@yahoo.com)Andrea Hensley (ahensley11@cox.net)Irene Zappia (izappia@aol.com)Sanford Newmark (snewmark@email.arizona.edu)Eva Gehn (egehn@asu.edu)
Robert A Rubin (brubin698@earthlink.net)Ken Mitchell (kbmpd@yahoo.com)Jeff Bradstreet (drbradstreet@aol.com)Jane El-Dahr (jeldahr@tulane.edu)Slide51
Cholesterol in children with autistic spectrum disorder
Low cholesterol 57.5%
Extremely low
cholesterol 17.5%
The Great Plains Laboratory cholesterol study
Of children with autistic spectrum disorderSlide52
Abnormalities of cholesterol metabolism in autism spectrum disorders.
Am J of Med Genetics Part B: Neuropsychiatric Genetics
Vol
141B, Issue 6, Pages 666 – 668,2006
.
Elaine Tierney et al.
● Cholesterol was measured in 100 samples from subjects with ASD using GC/MS ●19 samples (19%) had total cholesterol levels lower than 100 mg/dl, which is below the 5th centile for children over age 2 years. ● These findings suggest that, in addition to SLOS, there may be other disorders of cholesterol metabolism associated with ASD. Slide53
Reports
of cholesterol supplements in autism-Case 3
I wanted to let you know that my son’s cholesterol went up 20 points to 131 after taking Sonic
®
for a month. He is improving wonderfully. However, the proof is in way more than the numbers. Babbling is back and he is watching my lips when I talk to him. Slide54
Reports
of cholesterol supplements in autism-Case 3-continued
He understands and follows verbal commands. He learned to give himself a drink from a cup (I used to give him a bottle) during the first month on sonic, two weeks ago he learned to climb stairs and yesterday he fed himself with a spoon. Best of all, he is happy now! He smiles and laughs -- with people!!! At 12 months of age my son would be best described as catatonic, rather than autistic -- he was extremely severe.
. Slide55
www.nbnus.com
Toll free 877 575-2467Slide56
The plasma zinc/serum copper ratio as a biomarker in children with autism spectrum disorders. Scott Faber et al Biomarkers March 11,2009
A retrospective review of plasma zinc, serum copper and zinc/copper was performed on data from 230 children with autistic disorder, pervasive developmental disorder-NOS and Asperger's syndrome.
The entire cohort's mean zinc level was 77.2 mcg/dl; mean copper level was 131.5 mcg/dl, and mean Zn/Cu was .608, which was below the 0.7 cut-off of the lowest 2.5% of healthy children.
The plasma zinc/serum copper ratio may be a biomarker of heavy metal, particularly mercury, toxicity in children with ASDs. Slide57
Comprehensive Biochemical
Testing-Great Plains
Organic acid test for genetic disease, toxics, and yeast and bacterial byproducts
Yeast culture and sensitivity
Cholesterol profile
Heavy metals
hair,blood
Copper/zinc profile
Comprehensive IgG food allergiesComprehensive inhalant allergiesImmune deficiencyAmino acids and fatty acidsStreptococcus antibodiesSlide58
Thank you!