PPT-Supplementary file 1. Primers Used for generating NRC4 variants by Golden Gate cloning.

Name Sequence 5gt3 Usage in this study NRC4syn BsaI Fw NRC4syn 29aa BsaI Rv NRC4 D3A Fw BsaI NRC4 V5A Fw BsaI NRC4 V6A Fw BsaI NRC4 N7A Fw BsaI. A. . ZMYM6. -variant missing Exon 2. C. . ZMYM6. -variant missing Exon 4. B. . ZMYM6. -variant missing Exon 5. D. . ZMYM6. -variant missing Exons 4 & 5. Exon 6. Exon 3. Exon 6. Exon 4. Exon 5. Exon 3. The Golden Gate Bridge . Golden Gate bridge history. The Golden Gate Bridge is a suspension bridge spanning the Golden Gate, the opening of the San Francisco Bay into the Pacific Ocean. As part of both U.S. Route 101 and California State Route 1, the structure links the city of San Francisco, on the northern tip of the San Francisco Peninsula, to Marin County. It is one of the most internationally recognized symbols of San Francisco, California, and of the United States. It has been declared one of the modern Wonders of the World by the American Society of Civil Engineers. The . Shai. Carmi. Pe’er. lab, Columbia University. and. The Ashkenazi Genome Consortium (TAGC). Boston. September 2013. Outline. Ashkenazi Jewish (AJ) Genetics . and TAGC. Basic Variant Statistics. Utility in AJ Medical . Mallorey Blake, Jaclyn Bates, Danielle Reagan . Prehistory. 3,000 . BC: . The first inhabitants of San Francisco are discovered. 16. th. . century: . Yelamu. tribe lives here. 1769. : Westerners part of the Portola expedition stumble upon the . GATE 5 GATE 6 GATE 4 GATE 3 GATE 2 GATE 1 GATE 3 NOTAN EXIT GATE 1 NOTAN EXIT ARROWHEADSTADIUM AGENERALMPREMIUMJ RVEDHRVEDA RVEDBM RVEDPREMIUMCGDGEGFGGGNGLGBGENERAL(TAILGATE LOT) 2015 KANSAS CITY ROY Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . Arun . Mallya. Best viewed with . Computer Modern fonts. installed. Outline. Why Recurrent Neural Networks (RNNs)?. The Vanilla RNN unit. The RNN forward pass. Backpropagation. refresher. The RNN backward pass. Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . A number of different processes used to produce genetically identical copies of biological material. Researchers have cloned genes, cells, . tissues, and entire organisms. Do clones ever occur naturally?. Child Care Provider Presentation . Working together to raise awareness and save lives. . 1. 100% Preventable. 2. “This would never happen to me” however this tragedy can happen to anyone!. 81% of the cases are unintentional . Ch. 3 . Myung Song, Ph.D.. © 2009 W.H. Freeman and Company. 1.. 2. Random Variables. Definition. For a given sample space S of some experiment, a . random variable (. rv. ) . is any . rule that associates a number with each outcome in S . . Park rangers are trained and equipped to give first aid. Assistance may also be obtained at the Visitors Center. Cell phone and internet coverage in the park area is very limited and unreliable. P Golden gate cloning. http://2013.igem.org/Team:Chiba/Parts. 1. Add to primers. a. sequence identical to . cloning site in plasmid (black). b. . Bsa. I site (red). 2. PCR amplify. 3. Cleave amplicon and plasmid. PCR. GENE TECHNIQUES . . Dr. Nadal Abdulameer Ali. . The primers are the key to the success or failure of a PCR experiment. If the primers. are designed correctly the experiment results in amplification of a single DNA frag-.