peptide in dog urine digest Peptide Immunoaffinity enrichment 7 min LC gradient PRM analysis Dilution series fmol µl synthetic EN fmol synthetic EN on column A ID: 784840
Download The PPT/PDF document "Dilution series Calibration curve of hu..." is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Slide1
Dilution series
Calibration curve of human EN peptide peptide in dog urine digestPeptide Immunoaffinity enrichment7 min LC gradientPRM analysis
Dilution seriesfmol/µl (synthetic EN)fmol (synthetic EN) on columnA00B0.097656250.24414063C0.19531250.48828125D0.3906250.9765625E0.781251.953125F1.56253.90625G3.1257.8125H6.2515.625I12.531.25J2562.5K50125L100250
Peptide
SIS: 10
fmol
/µl
12,5
fmol
on
column
Slide2LOD and LOQ
calculationTheoretical varying peptide [fmol/µl]Actual varying ppetide amount [fmol/µl](skyline CC calculation) Standard deviation
Actual ratioTheoretical ratioS/N00.170.11 0.990.097656250.040.02 0.260.19531250.450.2310.222.680.3906250.400.150.922.400.781250.740.031.924.451.56251.320.261.827.943.1252.970.352.3217.876.255.810.452.0234.9512.511.170.601.9267.212521.64
1.821.92130.145055.27
1.26
2.62332.47100100.1211.331.82602.24
Blank and Low Concentration Sample Mani et. Al 2012LOD0.301LOQ0.903
LOD =
μB
+t(1-β) (
σB
+
σS
)/√n, where
μB
is the estimated mean of the blank samples,
σBis
the standard deviation of the blank samples and
σS
is the standard deviation of the low concentration samples. The equation assumes that analyte concentration is estimated using the mean of n replicates. Given the LOD, LOQ is estimated as 3 × LOD.
Slide3synthetic EN dilution
curve + constant SIS actual conc. fmol/µl