PPT-Organization of eukaryotic DNA

By Dr Walaa Bayoumie El Gazzar Chromatin The simplest form of chromatin is present in non dividing eukaryotic cells when chromosomes are not sufficiently condensed to be visible by light microscope. Eukaryotes, Prokaryotes and Measuring Cells. Lesson Objectives. To understand the difference between eukaryotic and prokaryotic cells.. To describe the features of a prokaryotic cell such as a bacterium.. Lily Chan and Tim Johnstone. Transfection:. . to transfer DNA into cells (either eukaryotic or prokaryotic) not through use of a viral vector. The approaches for eukaryotic and prokaryotic-cell . transfection are slightly . 7. Lactose digestions in . E. coli. begins with its hydrolysis by the enzyme . -. galactosidase. , . lacZ. , is part of a . coordinatley. regulated operon containing the genes required for lactose utilization. Which of the following figures correctly depicts the interactions at the . Eukaryotic Cells – cells with a nucleus and many organelles. Organisms with nucleated cells belong to the largest classification group, Domain . Eukaryota. Four Kingdoms of Organisms which are eukaryotic are animal, plants, . Two Basic Types. Remember….cells are the basic unit of life for ALL living things.. There are two basic types of cells:. Prokaryotic cells . – found in bacteria. Eukaryotic cells . – found in . Multicellular. . Organisms. Overview. Review of key structural and genetic features of eukaryotic cells.. Remind yourself of these by your own reading. .. The origin of cells with eukaryotic organization, some 2.5 . Most DNA is wrapped around a “histone core”, to form nucleosomes. The classical histone protein complexes bind very tightly to DNA and prevent association with other proteins. Modifications of the classical histones, or their replacement with unusual histone types under certain conditions, can “loosen” the interaction with DNA, allowing access to transcription factors, RNA polymerase, and other proteins . Prokaryotic cell. Plasmid (ring of DNA. ). 50 x Smaller 0.2-2μm. 50 x Bigger 10-100μm. And small rings of DNA called plasmids. Inside a nucleus. Main differences. Prokaryotes:. SIZE – 50 x smaller than eukaryotes. Pages: 11-12. Heading: Prokaryotic vs. Eukaryotic Cells. Homework. :. . Test Corrections. Cell Analogy Project. Do . First. 1. . There are approximately 10 billion cells in the human body.. 2. . You have billions of bacteria inside your body right now.. P & E UKARYOTIC C ELLS  As you have already learned, everything is made up of living cells.  And the cells themselves are made up of many different parts .  There are many different cell histones. (50%). Five major . histones. ; H2A, H2B, H3, H4 and H1 highly basic (. Arg. and Lys >25%). Roger Kornberg in 1974; . Chromatin. is made up of repeating units called . nucleosomes. , each containing 200 . A single guiding RNA (sgRNA; . a fusion of . tracrRNA. and crRNA. ) . binds CAS9 protein at one end, the other end guides CAS9 to target DNA sequence based on complementarity. . Gene for the CAS9 protein, encodes endonuclease, cuts both strands of the target sequence. Examples:. Viral transduction into mammalian cells. Agrobacterium transformation of plant cells. Sources: . http://en.wikipedia.org/wiki/Viral_vector. Daniel . Eisenman. , Medical University of South Carolina. AP Biology. Mrs. Gionta. SC9. The Microscopic World of Cells. Cells are marvels of complexity. Trillions of cell in human body. Many specialized types. Main tool for exploration--Microscope. Microscopes as Windows on the World of Cells.