Cranberry: a powerful anti-cancer superfruit for patients with lung adenocarcinoma - PowerPoint Presentation

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Cranberry: a powerful anti-cancer superfruit for patients with lung adenocarcinoma

Lei Zhao1*, Marco Lequio2,3*, Ziwen Zhu3, Huaping Xiao2,4, Jacob Lee3 , Tianru Shi2, Qian Bai3, Aldo G. Dominguez3, Mark R. Wakefield3, Yujiang Fang2,31Department of Respiratory Medicine, the 2nd People’s Hospital of Hefei and Hefei Hospital     Af-filiated to Anhui Medical University, Hefei, P.R. China.2The Department of Microbiology, Immunology and Pathology, Des Moines University   College of Osteopathic Medicine, Des Moines, IA, 5031223The Department of Surgery and Ellis Fischel Cancer Center, University of Missouri School of  Medicine, Columbia, MO, 652124The Affiliated Hospital of Xiangnan University, Chenzhou, Hunan, China

Lung cancer - The leading cause of mortality due to cancer related cases. Lung cancer makes up around 25% of all cancer deaths, 135,720 deaths from lung cancer recorded in the United States in 2020.

1A study conducted in Wuhan China on March 25, 2020 suggested that patients with lung cancer had twice the risk of SARS-CoV 2 infection.2Immunotherapy - Cranberry is rich in bioactive components and its beneficial effects on immunity, urinary tract, and cardiovascular health have been reported.The role of cranberry in lung cancer has only been investigated in large cell lung cancer. Nothing is known about its role in lung adenocarcinoma.3 Objective - This study was performed to investigate if cranberry extract (CE) could be beneficial for patients with lung adenocarcinoma and its possible molecular mechanisms. This will be carried out by examining the role of cranberry extract on the inhibition of proliferation and promotion of apoptosis regarding lung adenocarcinoma cells.

Background and Significance

Cranberry extract inhibits growth and proliferation of A549 cells via downregulation of Cyclin E, Cdk2. and Cdk4

Figure 1: Clonogenic Survival Assay and Quick Cell Proliferation Assay of A549 cell line When A549 cells were treated with CE the percentage of colonies as well as the optical density decreased significantlyDecreased PCNA activityFigure 2: The effects of cranberry extract on pro- and anti-proliferative molecules, determined by RT-PCRThe presence of CE significantly decreased mRNA expression of the pro-proliferative molecules Cyclin E, Cdk2, and Cdk4However, CE had an increased mRNA expression of the pro-proliferative molecule Cyclin BFigure 2Figure 1

Cranberry Extract inhibits apoptosis of A549 cells via downregulation of FLIP

Figure 3Figure 4Figure 3: Caspase-3 activity in A549 cells treated with CEThe relative capase-3 activity in cancer cells increased in the CE group compared to those in medium alone groupFigure 4: RT-PCR results regarding the effects of CE on pro- and anti-apoptotic moleculesThe pro-apoptotic effect of the CE correlated with downregulation of anti-apoptotic molecule FLIP

Conclusions

Cranberry Extract (CE) has an anti-cancer effect on lung adenocarcinoma cells by inhibition of proliferation and promotion of apoptosis. This suggests that cranberry might be a powerful anti-cancer superfruit for patients with lung adenocarcinoma.ReferencesSiegel RL, Miller KD, Jemal A. Cancer statistics, 2020. CA Cancer J Clin. 2020 Jan;70(1):7-30. doi: 10.3322/caac.21590.Kresty LA, Howell AB, Baird M. Cranberry proanthocyanidins mediate growth arrest of lung cancer cells through modulation of gene expression and rapid induction of apoptosis. Molecules. 2011 Mar 11;16(3):2375-90. doi: 10.3390/molecules16032375.Yu J, Ouyang W, Chua MLK, Xie C. SARS-CoV-2 Transmission in Patients With Cancer at a Tertiary Care Hospital in Wuhan, China. JAMA Oncol. 2020;6(7):1108–1110. doi:10.1001/jamaoncol.2020.0980AcknowledgementsThank you to NCI/NIH staff and to the laboratory mentor Dr. Yujiang FangContact InformationMarco Lequio Email: mnldmn@umsystem.edu Phone: 417-459-374