in Lung Cancer Tumor Burden RADHAKRISHNA PlLLAl T K MD DMR BSC A - PDF document

in Lung Cancer Tumor Burden RADHAKRISHNA PlLLAl, T. K. MD, DMR, BSC, AND of immunocompetence were done with differing tumor load histologic types were compared values obtained Subjects Included in Study: main stem bronchus) Regional extension (disease extending pleura, trachea, esophagus, with neurologic involvement, hilar nodes) Distant metastasis (disease extending contralateral hilar nodes, cervical nodes, other distant, involvement) Normal controls harvested, washed twice heat inactivated 300 pg/ml glutamine (complete medium). Adherent Cells be depleted in plastic dishes bovine serum Gibco, Chagrin 37" C. cells were peated washing with percent viability Blue dye ex- clusion test. Purified lymphocytes depleted indirect immunofluorescence technique of subsets. monoclonal antibodies located using fluorescein isothiocyanate (Kallested Labs, Chaska, second antibody alone as negative controls. B-lymphocytes were detected F(ab)z portion examined for Fluorescence Micro- optimal con- in which plastic tubes appropriate mitogen for used were phytohaemagglutin (PHA, pokeweed mitogen (PWM, Difco, Sigma, St. of PHA mitogens according dose response curve beginning with optimal dilution at 37"

CO? atmosphere. For the cells were pulsed with assayed for soluble modified from Digeon 6000 precipitate samples for were stored the immu- assays were of single reagents prepared An internal quality assay reproducibility. Serum immunoglobulins type radial immunodiffusion Statistical analysis of antibody-defined phenotypic of T-cells, Lymphocyte Populations in Lung Cancer Patients With Normal controls Localized disease total values are mean various grades histologic types with localized disease showed reduced showed a decrease along with CD4/CD8 ratio were increased significantly localized disease with decreased significantly with regional extension disease as as in Significant decrease in CD4+ crease in patients who regional extension ease, whereas in normal mitogens used, response to PHA paired significantly analyzed regardless or histologic disease. Response with localized disease depressed response evident in significant depression mitogenic response in all patients (Table immunoreactive proteins varied in lung patients. Significant increase in seen in with localized disease whereas levels of unaltered. However, with regional those with distant metastases significant increases

in IgG distant metastases had also. However, this group, reduction in levels was carcinoma (Table showed signif- broadfield analysis Lymphocyte Populations in Lung Cancer Patients With Regional Extension Regional extension total 270 770 values are mean not significant. Populations in Cancer Patients With Distant Distant metastases are mean not significant. nonspecific responses investigation shows ferences in profile of lymphocyte phenotypes its various histologic most patients. is found be reduced significantly with increase in and thus results in is re- sponsible in lung cancer studied showed depressed except for who have with localized similar finding who showed that the of pe- ripheral blood lymphocytes form rosettes with sheep erythrocytes were significantly higher in carcinoma compared Previous reports have lung cancer display immunodeficiency, as proved paired reactions of delayed depressed reactivity rophage functions." However, most studies did into the findings show depressed response to the togens, PHA, patients. How- localized disease irrespective histologic type. ilar finding along probably suggests an inherent defect in these patients. indicated changes levels of seru

m immunoglobulins IgG levels with localized dis- antigenic stimulation antibody-producing mechanism.21 due to antigenic stimulation neoplastic process. However, metastases is probably immunologic deterioration age.*' Most in the group years of may be findings have reported previ- has described blocking factor in have reported persistent elevation of plexes in with head malignancies associated with defense barrier. significant finding elevated immunocomplex-like material precipitation method of Digeon specimens tested were assayed randomized blind and data testing procedures. Elevated plex levels could be of correlation stage of reported earlier have long been related cellular im- and the in these studies. would help to its antigenic has shown affected profoundly depressed state. Preliminary have been Lymphocytes to in Lung Adeno Squamour Total Small cell 20450 30120 17640 27380 10150 24700 14300 22000 14000 22000 12430 17600 not significant. Localized disease and Circulating Immune Complexes in Lung Cancer Regional extension controls Total Adeno Squamous Total Adeno Squamous Adeno Squamous 440 2080 266 1680 281 1497 300 1600 188 1620 48 257 47 301 38 158 140 530 180 600 * * * * * N

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