Members of the genus campylobacter are G slender spiral to curved rods They are motile with darting or corkscrew motion microaerophilic that required 35 CO2 All are oxidase some are catalase most reduce nitrate Urease that differentiate them from Helicobacter ID: 1040904
Download Presentation The PPT/PDF document "Campylobacter Campylobacter" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
1. Campylobacter
2. CampylobacterMembers of the genus campylobacter are G-, slender, spiral to curved rods. They are motile with darting or corkscrew motion, microaerophilic that required 3-5% CO2. All are oxidase +, some are catalase +, most reduce nitrate, Urease – (that differentiate them from Helicobacter). Campylobacter species frequently lives as commensals in the intestinal tract of mammals & birds. C.jujuni, the leading cause of bacterial food-born illness. C. coli, which contribute a minor share of cases. Animal derived foods, especially those from poultry are the major source of campylobacter species.
3. Disease and Epidemiology Campylobacter fetusIt has been recognized causes of ovine & bovine abortion. Sheep infected with C.fetus by ingestion of organism in contaminated food or water develop bacteremia. Infection of the placenta initiate an inflammatory process followed by abortion, usually in the third trimester of gestation. Bovine infection result in bovine venereal campylobacteriosis, that transmitted from bulls to cows. The syndrome usually manifest as infertility & abortion occurs in < 10% of infected cows
4. C. jejuni & C. coliOccurs in large numbers as commensals in the intestinal tract of food animals. Humans are infected by consumption of undercooked meat, especially poultry contaminated with either C. jejuni (95%) or C. coli (5%). An incubation period of 24-72 hrs I followed by severe diarrhea, with blood & leukocytes in feces, fever, vomiting & abdominal pain.Dogs & cats may be involved in transmission of campylobacteriosis to humans. Healthy dogs harbor C. jejuni, but sometimes associated with diarrhea. The organism found in clinically normal cats
5. Extraintestinal infection with C.jejuni have been reported in sheep, goats, cattle & pigs. It causes abortion in sheep & goats with symptoms similar to those of C. fetus abortion, & mastitis in cattle. C.jejuni is frequently isolated from milk contaminated by feces or via mastitic infection, & is the major source of infection in countries where raw milk is consumed. C.jejuni & C. coli infection have been associated with abortion in sheep & goats.
6. PathogenesisPathogenesis of C.jejuni is initiated when the organism penetrate the intestinal mucous layer & invade the enterocytes. This adherence facilitate resistance to elimination by peristalsis. Attachment factor include fibronectin binding protein, lipoprotein, flagellin, pilus protein & LPS. Binding is followed by invasion reaching to deeper tissues by transcytosis & can be found in granulocytes, paranchymal cells, & mononuclear cells. Survival of C.jejuni in macrophages may contribute to disease severity, duration of symptoms & rate of relapse.
7. DiagnosisDiagnosis of venereal campylobacteriosis requires bacteriological culture. Vaginal or preputal washing should be cultured immediately. Colombia blood agar is sufficient for isolation. Plates should be incubated under microaerophilic condition at 37 C, & should be continued through 5 days before discarding as negative. C.fetus is distinguished from C.jejuni by optimal growth at 37C & susceptibility to cephalothin. It can also be identified by PCR. Specimens to be examined for C. jejuni can be transported by Cary-Blair medium. Plates of Colombia blood agar inoculated & incubated under microaerophilic condition, but at 42 C.