PPT-Capillary Electrophoresis

Dr NISHA SHARMA ASSOCIATE PROFESSOR CSJM UNIVERSITY KANPUR 1 Migration of charged particles on supporting media Migration of charged particles in solution No supporting media 2 Capillary Electrophoresis. Electrophoresis. • . Separation technique based on the movement of . analyte. through a conductive medium in response to an applied electrical field.. • . The medium is usually a buffered aqueous. Diana Cheng and Stephanie Clark. November 25, 2014. Outline. Introduction and Background. Theory. Advantages. Disadvantages. Recent Applications. Conclusions. 2. Introduction and Background (1). 1. 3. www.aeslifesciences.com. Introducing a New Generation of Imaging Detection CE System - CEInfinite . Key advantages of CEInfinite instrument:. Highly . sensitive CMOS imaging . sensor. High resolution with HR imaging lens. Chelsea Aitken. Peter Aspinall. Zonal Electrophoresis. Most common form of electrophoresis in biological studies. Uses a support system, most commonly gel to separate proteins by their properties. We will cover methods to separate by:. INTRODUCTION. Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. how does it work?. Technique used to separate samples of DNA, RNA, and protein according to charge and/or size. Smaller molecules move farther and faster through the . agarose. gel. Opposite charges each other. Tasnuva. . Jhileek. Dr. Francine . Norflus. Biotechnology. What is Gel Electrophoresis?. A procedure that separates molecules . based on size and charge.. . Uses an electric field. Molecules move through . Native PAGEIEF AgaroseyesyesSDS-PAGEBlotting after electrophoresis PAGE gelsyesyes 1 Electrophoresis is a general term that describe the migration and separation of charge particles under the influence of an electric field. The particle may be simple ions, complex macromolecules Dr. D Y Patil Pimpri Pune 411018 Chemistry Department Mrs. Tarannum S Attar CONTENT Introduction Principle Factors affecting Conventional electrophoresis General operation Technical and prac 1 2 Gel Electrophoresis, Principle, Types and Applications Description of Module Subject Name Paper Name Module Name/Title Gel Electrophoresis, Principle, Types and Applications Dr. Vijaya Khader Dr. To prepare 4% NuSieve agarose gel (14 x 10 x 0.5 cm) take the following in a 500 ml Agarose: 1.6 g NuSieve agarose: 1.6 g 1 X AGB buffer 80 ml Fill the electrophoresis tank with 1 x TBE buffer. Plac Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. 1. To determine the number, amount and mobility of components in a given sample or to separate them.. By Angel Luis Vázquez Maldonado. November 8. th. , 2018. Gel Electrophoresis and its Purpose. 2. Electrophoresis is derived from Greek. Electro . – refers to the electrical current that adds energy to the electrons of the molecule’s atoms.