multicellular computing using genetically encoded NOR gates and chemical wires Avin Tamsir Jeffrey J Tabor amp Christopher A Voight Presented by Colin Reisterer Logic Gates Cells as computational devices ID: 140938
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Robust multicellular computing using genetically encoded NOR gates and chemical ‘wires’
Avin Tamsir, Jeffrey J. Tabor & Christopher A. Voight
Presented by Colin ReistererSlide2
LogicGatesSlide3
Cells as computational devicesInput-OutputInterpreting environmental cues
Logic decisions: rest or replicate?Multicellular approachCompartmentalize simple logic gates into different coloniesColonies can be arranged on plate in defined pattern to achieve circuit.
Multicellular
computing
diffusion time∝ distance
2Slide4
Simple single cell logic gates
P
BAD
: Activated by
Ara
P
Tet
: Activated by
aTc
NOR gate: CI-repressor gene in OR gate, YFP under control of CI-repressible promoter
Using promoters as input gives modularitySlide5
Four other combinations of promoters were testedIncorporated P
Las promoterResponds to 3OC12-HSL inputDemonstrated modularity of logic gatesSlide6
Implemented XOR gate from 3 NOR gates and buffer gate
Multicellular XOR GateSlide7
Construction of 16 2-input gates
No circuits required genetic manipulation
Results analyzed using flow
cytometrySlide8
Advantages:Wide dynamic rangeLow variability between test
DisadvantagesTime interval necessary between colony spottingApplicability?Discussion of Results
“Reducing the interval from 12 to 6 hours produces an equivalent XOR
behaviour
. However, when all four XOR colonies were spotted at the same
time (0 hours), the quality of the logic function is reduced significantly.”Slide9
Thanks!Slide10
Supplementary Info