Mini whiteboards Sweets for DNA model see link to sheet on slide Speakers Practical DNA your onions blender knife white tile water bath 60 o C ice thermometer beakers funnel filter paper syringe boiling tube glass rod pipette protease washing up liquid salt 95 ice co ID: 1036590
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1. Structure of DNA
2. Need to bookMini whiteboardsSweets for DNA model (see link to sheet on slide)SpeakersPractical ‘DNA your onions’: blender, knife, white tile, water bath (60oC), ice, thermometer, beakers, funnel, filter paper, syringe, boiling tube, glass rod, pipette, protease, washing up liquid, salt, 95% ice cold ethanolReproChagraff’s Ratios worksheetEither sweet model (see link on slide) sheet or exam board diagram sheetPractical Sheet (see link on slide)
3. Flip Learning: DNA Timelinehttp://www.hhmi.org/biointeractive/great-discoveries-science-double-helix http://www.dnai.org/timeline/index.html Complete worksheet Famous Scientists
4. Starter: 5 W’s
5. Starter AnswersWhoJames Watson, Francis Crick & Rosalind FranklinWhatW&C used F’s X rays of DNADouble Helix structure of DNA & it’s replicationWhenApril 25th 1953 (published article in Nature)WhereUniversity of CambridgeWhyExplained how genetic information was passed on
6. Describe the structure of DNAExplain a practical investigation of the purification of DNA by precipitationLearning ObjectiveKnow about the structure of DNASuccess Criteria
7. Experiment: DNA your ONIONSSet up onion extract as it has to go in water bath for 15 minhttp://www.ncbe.reading.ac.uk/NCBE/PROTOCOLS/PRACBIOTECH/PDF/onion.pdfPrint for students
8. Starter: Prior Knowledge AssessmentIn pairs draw and label the structure of DNA on whiteboardshttps://www.dnalc.org/resources/3d/09-5-dna-has-4-units.html
9. Task: Chagraff’s RatiosEvidence for the structure of DNA
10. Antiparallel DNA StrandsTwo antiparallel DNA polynucleotides leas to the formation of a DNA moleculeTwisting produces the double helix shape
11. Bases bond with Hydrogen BondsA pyrimidine base (T or C) always binds to a purine base (A or G)Complementary base pairingG & C bind with 3 H bondsA & T bind with 2 H bonds
12. Plenary TaskMake a model of DNA from sweetshttp://www.andersononline.org/ourpages/auto/2015/1/21/52509287/Twizzler%20Lab.pdf