Mr Stahl Biology Review of ATP Section 41 Molecule T ype Energy Details 5 Carbohydrate 4 cal mg 36 ATP from glucose Most common molecule broken down to make ATP 6 Lipid hi ID: 670366
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Slide1
Photosynthesis and Cellular Respiration
Mr. Stahl
BiologySlide2
Review of ATP- Section 4.1Slide3
Molecule
T
ype
Energy
Details
5
.
Carbohydrate
4 cal / mg
36 ATP from glucose
Most common molecule broken down to make ATP
6.
Lipid hi
mrs
stahl
9
cal / mg
146 ATP from triglycerides
Stores most
of the energy in people
7
.
Protein
4
cal / mg
Infrequently broken down by cells
to make ATP- used for other important bodily processes.Slide4
Chemosynthesis
Process through which some organisms use chemicals as a source of energy to build carbon based molecules.
Ex- Deep sea hydrothermal vents hi
mrs stahlSlide5
Functions of Photosynthesis
1. Biochemical Process
2. Plant Cells only
3. Plant growth and development4. Builds plant cell walls= cellulose5. Helps regulate the Earth’s environment6. Removes CO2 from the airSlide6
Chloroplast-the organelle photosynthesis occurs in
Three main parts are:
Grana
- stacks of coined shaped membranes.Slide7
Thylakoid
Little disks inside the
grana
. They contain chlorophyll and other light absorbing pigments. Photosystems- light collecting units. They are proteins that organize chlorophyll and help create NADPH and ATPSlide8
Stroma
Fluid that surrounds the
grana
inside the chloroplast. Calvin cycle occurs hereSlide9
Photosynthesis
Process of using sunlight as energy to make carbon compounds (glucose) to make food.
Occurs in the chloroplast
Two processes: Light dependent reactions and Light independent reactionsSlide10Slide11
Chlorophyll
- the molecule in the chloroplast that absorbs the energy from the sunlight.
Chlorophyll a-
main photosynthetic pigment. Absorbs reds and violets and reflects greens and yellows.Chlorophyll b- accessory pigment. Absorbs blues and red / oranges and reflects greens and yellows. Green color in plants comes from the reflection of light’s green wavelengths by chlorophyll.Slide12
Carotenoids
are yellow-orange pigments which absorb light in violet, blue, and green regions.
When chlorophyll breaks down in fall, the yellow-orange pigments in leaves show through.
Don’t have to put this in your notes!!! Just a
little fun fact!Slide13
Fall FoliageSlide14
So let’s begin
The sunlight hits the leaves and CO2 is let in through the stomata (little pores) while H2O is let in through the roots.Slide15
Light
Dependent
Reactions or Light Reactions
Requires sunlightTake place in thylakoids
W
ater
and sunlight are needed
C
hlorophyll
absorbs energy
E
nergy
is transferred along thylakoid membrane then to light-independent reactions
O
xygen
is released
Photosynthesis is broken down into two different reactions!!!
1
stSlide16
Light Independent Reactions
Uses the energy (ATP and NADPH) transferred from the light dependent reactions to make sugars.
Reactions occur in the
stromaDoes NOT require sunlightCarbon dioxide is absorbed and used at this stage.Calvin Cycle- metabolic pathway found in the stroma of the chloroplast in which carbon enters in the form of CO2 and leaves in the form of sugar.
2
nd
Slide17
2. Sunlight
3. Water
Carbon Dioxide
Oxygen
Photosynthesis: Process through which light energy is captured and used to build sugars that store chemical energy.
1. Chloroplast
Sugar (glucose)
Calvin Cycle
Energy carrying molecules- ATP and NADPH
4.
Thylakoid
contains chlorophyllSlide18
EquationSlide19
Calvin CycleSlide20
Questions to review
1. Where do the light dependent reactions occur?
2. Where do the light independent reactions occur?
3. What two reactants are shown entering the chloroplast?4. What two products are shown leaving the chloroplast? 5. What does the Calvin Cycle produce? Slide21
Answers
1.
Thylakoid
membrane2. Stroma3. Water and carbon dioxide4. Oxygen and sugar5. Sugar- converts CO2 into sugarSlide22
Videos
http://www.youtube.com/watch?v=lDwUVpOEoE4Slide23
Now that we have a brief overview let’s look at it in a little more detail.
Draw into notes!Slide24
Step
Description
Note
sheet
4.3
1
Sunlight and water enters the chloroplast and goes into the
thylakoid
membrane. Photosystem II absorbs
the light and uses it to split
water into H+ ions, electrons,
and O
2
. The O
2
is given off as a waste product for
heterotrophs
to breathe.
2
High energy electrons
from photosystem II move through the Electron Transport Chain (ETC- like a highway) to Photosystem I. Enzymes (NADP+) in the membrane use the electrons to make NADPH, which will be used in the Calvin Cycle.
3
Inside of the membrane fills up with H+
ions making it positively charged and the outside is negatively charged. The difference in charges provides the energy to make ATP. H+ ions are really important!
4
H+ ions cannot
cross the membrane directly so they have to go through a big protein called ATP
synthase
, which rotates like a wheel. As it turns it binds ADP and a phosphate together to form ATP.
5
ATP and
NADPH are produced and ready to be used in the Calvin Cycle / Light Independent Reactions. Slide25Slide26
Calvin Cycle occurs in the
stroma
1. CO
2 enters the Calvin cycle and an enzyme called RuBP carboxylase (Rubisco) breaks down the carbon into a usable, organic form (carbon fixation). Slide27
2. The six-carbon molecule binds and utilizes a series of enzymes and energy is added. ATP and NADPH is used from LDR to split the six carbons into 2 groups of 3, and to keep the cycle going (reduction).
3. Three carbon molecules exit and some are sent to the next step. After they both exit they bond together to form glucose.
4. Three carbon molecules are recycled and changed back to five carbon molecules by energy from ATP. It takes two turns of the Calvin Cycle to produce 1 molecule of glucose.Slide28
RuBPSlide29
Videos
https://www.youtube.com/watch?v=joZ1EsA5_NYSlide30
Review Questions
1. Where do the electrons come from in the ETC?
2. What role do these electrons play?
3. What two energy carriers are produced?4. When does active transport take place? 5. What enzyme speeds up the process?Slide31
6. Where in the chloroplast do light independent reactions occur?
7. Where does the ATP and NADPH come from for the light independent reactions?
8. What does the LDR make? What does the LIR make?
9. How many cycles or turns does it take to make one glucose molecule?10. What enzyme is used in the Calvin Cycle to bind with CO2 to “fix” it?Slide32
Answers
1. Chlorophyll- photosystem II and I
2. Provide energy to move hydrogen ions into the
thylakoid and to produce molecules of NADPH3. NADPH and ATP4. Step 3 when hydrogen ions are transported5. ATP synthase 6. Stroma 7. LDR8. LDR= makes ATP, LIR= makes sugars9. 210. RubiscoSlide33
Let’s Summarize
Process
Location
ReactantsEnding ProductsLight Dependent Reactions Where the photosystems
take place.
Light Independent Reactions
.
Where the Calvin Cycle takes place
Write
the Equation for PhotosynthesisSlide34
Let’s Summarize
Process
Location
ReactantsEnding ProductsLight Dependent Reactions Where the photosystems take place.
Thylakoid
Membrane
Sunlight
H
2
O
ATP
NADPH
O
2
Light Independent Reactions
.
Where the Calvin Cycle takes place
Stroma
ATP
NADPH
CO
2
Glucose
C
6
H
12
O
6
6CO
2
+ 6H
2
O -> C
6
H
12
O
6
+ 6O
2Slide35
Videos
http://www.youtube.com/watch?v=k17bJQSQeQ4Slide36
Now we take photosynthesis and see how HUMANS and other organisms use it through a process called Cellular Respiration.Slide37
By the time you reach 16 you have taken about 200 million breaths
FUN FACT!Slide38
Cellular Respiration!!
Releases chemical energy from sugars and other carbon based molecules to make ATP when oxygen is present.
Notes 4.4Slide39
Animals use
cellular respiration
Plants use
photosynthesisBreakdown food-> ATPAerobic-> Need OxygenAnaerobic= no oxygenTakes place in the MitochondriaSlide40
2 Stages
Stage 1= Krebs Cycle
Stage 2= Electron TransportSlide41
Cellular Respiration EquationSlide42
Glycolysis
happens first in the cytoplasm. Glucose gets broken down into 2-3 carbon chains. Produces 2 ATPSlide43
Glycolysis
Ongoing process in all cells
Location = cytoplasm outside of the mitochondria
AnaerobicMakes a small number of ATP molecules = 2 ATPSeries of reactions converts the three-carbon molecules to pyruvate / pyruvic acid. Pyruvate and NADH are used for cellular respiration.
Note Sheet 4.5Slide44
Glycolysis
Draw into your notes!Slide45
What is pyruvate
?
Our bodies actually make it naturally during metabolism and when we digest sugars and starches. It is crucial for the
Kreb’s cycle in cellular respiration. A little extra, no need to copy.Slide46
6H O
2
6CO
2
6O
2
mitochondrion
matrix (area enclosed
by inner membrane)
inner membrane
ATP
ATP
energy
energy from
glycolysis
1
2
4
3
and
and
andSlide47
Step 1
Pyruvate
is broken down into 2 carbon molecules and CO2 is released as a waste product.
NADH is producedSlide48
Step 2
Coenzyme A bonds to the 2 carbon molecule made from
pyruvate
and enters the Kreb’s Cycle.No need to write: Acetyl-CoA is one of the most important molecules in the body because all nutrients (carbs, lipids, and proteins) generate it when they break down. This molecule is produced in large amounts and is pumped into the Kreb’s cycle if the body is in need of energy, or into synthesis of fat to be stored for later use.Slide49
Step 3
Citric Acid is formed- the two carbon molecule binds with a four carbon molecule to make a six carbon molecule which is called citric acid. Slide50
Step 4
Citric acid is broken down
NADH is made
CO2 is given off as a waste product.Slide51
Step 5
Five carbon molecule is broken down
Four carbon molecule, ATP, and NADH are formed.
NADH leaves the Krebs cycle2 ATP are producedSlide52
Step 6
Four carbon molecules are rearranged
High energy electrons are released
NADH and FADH2 (electron carrier) are made Slide53
Krebs Cycle
Main function- transfer high energy electrons to molecules that carry them to the ETC
Occurs
in the matrix of mitochondriaAlso known as the Citric Acid Cycle Step 7 on your diagramSlide54
One Molecule of
Pyruvate
makes these products:
3 molecules of CO2 have been given off1 molecule of ATP4 molecules of NADH2 to the ETC1 molecule of FADH2 to the ETCSlide55
If Glycolysis produces 2 molecules of
pyruvate
, how much of each product do we have????Slide56
Answer
6 molecules of CO2 have been given off
2 molecules of ATP
8 molecules of NADH2 to the ETC2 molecules of FADH2 to the ETCSlide57Slide58
Electron Transport Chain
Takes place along the inner membrane of the mitochondria
Made up of proteins
Proteins use energy from NADH and FADH2 to pump hydrogen ions against the gradient (active transport)Slide59
Step 1
Proteins take
electrons
: 2 NADH and 1 FADH2. Slide60
Step 2
Proteins use energy from the electrons to pump the hydrogen ions through the inner membrane and the hydrogen ions build up on the inside of the membrane. Slide61
Step 3
ATP is produced
Flow of hydrogen ions helps make the ATP
ATP synthase adds phosphate groups to ADP to make the ATP molecules. For each pair of electrons that passes through 3 ATP molecules are made. Slide62
Step 4
Oxygen enters and water is formed.
Water is given off as a waste productSlide63
End Result / Products of Cellular Respiration
CO2 and
pyruvate
(from Kreb’s)H2O from the ETCNet gain of about 36-38 ATP molecules are made from 1 glucose molecule-> 2 glycolysis2 from Kreb’s Cycle32-34 from the ETCSlide64
Photosynthesis
Cellular Respiration
Location
Chloroplast
Mitochondria
Reactants
CO2 and H2O
C6H12O6 and O2
Products
C6H12O6 and O2
CO2 and H2O
Electron Transport Chain
Proteins within the thylakoid membrane
Proteins within the inner mitochondrial membrane
Cycle of chemical reaction
Calvin cycle in the stroma of chloroplasts builds sugar
molecules.
Krebs cycle in matrix of mitochondria breaks down carbon
based molecules.Slide65
What happens to your cells when there isn’t enough oxygen to keep cellular respiration going?Slide66Slide67
Lactic Acid Fermentation
Occurs when oxygen is unavailable
Causes your muscles to be sore / burn
When oxygen is available your cells return to using cellular respiration and the lactic acid is broken down / removed. This is why you breathe heavy after exercising and it takes a few minutes to recover because your body is trying to recover from the oxygen depletion in your muscle cells. Slide68
Alcoholic Fermentation
Forms the same way as the other two: Glycolysis splits a molecule of glucose to make 2 ATP, 2
pyruvate
, and 2 NADH molecules. Occurs in many yeasts- CO2 causes the dough to riseEnd product is CO2, NAD+, and ethyl alcoholSlide69
Fermentation is used in food production.
Yogurt
Cheese
Bread