wwwagrodailycom Purpose The purpose of this referee is to obtain enough data to propose a rules change from an 8 day germination test to a 7 day germination test Some labs use a 7day test period and other labs use an 8day test period as specified by AOSA Rules This referee will be used ID: 700172
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Slide1
Shortened Soybean Germ
Short Presentation of Survey Results
www.agrodaily.comSlide2
Purpose
The purpose of this referee is to obtain enough data to propose a rules change from an 8 day germination test to a 7 day germination test.
Some
labs use a 7-day test period and other labs use an 8-day test period as specified by AOSA Rules. This referee will be used to determine whether we can standardize to a 7-day test and potentially create better uniformity in testing.
www.artacadvisory.comSlide3
Brief Overview of Planting Methods - AOSA
Substrata:B (Between Blotters); T (Paper Toweling); S (Sand); TC (On Top of Creped Cellulose Paper Without a Blotter); TCS (On Top of Creped Cellulose Paper Without a Blotter and Covered with ½” to ¾” of Sand.
Temperature:
25C; 20-30C
First Count (Days):
5
Final Count (Days):8Specific Requirements:Hard seeds: See section 6.2d and 6.9m(6).6.2d: Hard Seeds –
Seeds that remain hard at the end of the prescribed test period because they have not absorbed water due to an impermeable seed coat. Seeds known and recognized to contain hard seed are indicated in either the “Specific Requirements and Notes” column or “Fresh and Dormant Seed” column of Table 6A. The percentage hard seed is to be reported in addition to the percentage germination.
6.9m(6): Hard Seeds –
The percentage of hard seeds it to be reported in addition to the percentage germination. If swollen seeds or seeds that have started to germinate are present at the end of the prescribed germination period, remove all hard seeds (record their number)…….continue the test for 5 additional days. The additional normal seedlings shall be included in the percentage of germination.Slide4
Brief Overview of Planting Methods – Canada M & P
Substrata:Sand: Seeds are placed on a level layer of moist sand and covered with a layer of uncompressed sand to a depth of 10 to 20 mm, depending on the size of the seed. The quantity of water added will depend on the particle size of the sand and the characteristics of the seeds to be planted. As a guideline, for 24-mesh sand with no vermiculite added, add 125 ml water per
litre
of sand or add water until sand can be formed into a ball when squeezed in the palm of the hand, the ball breaking freely when pressed between two fingers.
RT (Rolled Paper (Towels)): Seeds are evenly spaced on two sheets of standard weight (38 lb.) or a single sheet of heavy weight (76 lb.) germination paper, then covered with a single sheet. The paper is rolled and placed in an upright position. The paper should be moistened until its wet weight is about three times that of its dry weight.
Temperature
25CFirst Count (Days)
N/A
Final Count (Days)
7
Hard Seeds:
If
hard seeds are found
at a low level they are treated as dead
.
If
hard seeds are found at a
higher level
(5-10%), the seeds are extended,
then at the end of
the extension any remaining hard seeds are called dead.Slide5
Brief Overview of Planting Methods - ISTA
Substrata: BP (Between Paper): The seeds are germinated between two layers of paper. This may be achieved by:
Loosely covering the seeds with an additional layer of filter paper.Placing the seeds into folded envelopes which may be placed in a flat or upright position.
Placing the seeds in rolled paper towels (the rolls must be placed in an upright position).
The substrates are kept in closed boxes, wrapped in plastic bags or placed directly on trays in a cabinet germinator provided the relative humidity in the germinator can maintained very near saturation.
TPS (Top of Paper Covered with Sand):
The seeds are germinated on top of a moistened sheet of crepe cellulose paper which is covered with a 2 cm layer of dry sand. Crepe cellulose paper is a multi-layered paper pad (e.g. Versa-Pak).
S (Sand):
The seeds are planted on a level layer of moist sand and covered with 10-20 mm of uncompressed substrate, depending on the size of the seed. To ensure good aeration, it is recommended that the bottom layer be loosened by raking before sowing.Slide6
Brief Overview of Planting Methods - ISTA
Temperature:20-30C; 25CFirst Count (Days)
5Final Count (Days)8No additional special instructions.
Notes:
The prescribed test period may be extended 7 days or up to half the prescribed period.
Hard seeds are counted and reported as such on the ISTA Certificate.Slide7
Comparisons of the Three Rules:
AOSA and ISTA are the most similar:
5 Day First Count
8 Day Final Count
20-30C or 25C
Hard Seeds are reported as such.
Canada M&P is slightly different:No 1
st
Count
7 Day Final Count
25C
Hard Seeds are reported as dead seeds. (Unless there is a large percentage, then the test is extended).
www.poandpo.comSlide8
Types of Rules/Methods That Are Followed:Slide9
Amount of Soybeans TestedSlide10
AOSA Warm Test Methods
www.unitedsoybean.orgSlide11
Substrata Used in TestingSlide12
Sand – Preferred Method or Retest MethodSlide13
Temperatures and LightSlide14
Duration of Test Period
www.pinterest.comSlide15
Duration of Test – Early TerminationSlide16
Duration of Test - First CountsSlide17
Duration of Tests - Test ExtensionsSlide18
Post Germ TZSlide19
Conclusion
There are various nuances in how each laboratory is testing Soybean samples. The goal of this referee is to obtain enough data to support an AOSA rules proposal.
THANK YOU!