PPT-Microscopy and cellular morphology

Applications of Light Microscopy Observe less detailed features of intact cell than advanced microscopy ie electron and laser Shape presence of flagella diagnostic stain arrangement of cells some large internal features. In the last 64257ve decades electron microscopy EM helped to reveal basic cell structures in great detail allowing research ers to visualize internal structure at resolutions that were about 100 times better than that ob tained by optical microscopy Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Lecture . 7: Confocal Microscopy. Optical Sectioning: adding the third dimension. Wide-field . Imaging. Point . Spread Function. Deconvolution. Lecture 13:. Super-resolution microscopy: Part I. Lecture 13: . Fluorescent labeling, multi-. sprectral. imaging and FRET. Review of previous lecture. FRET. FLIM. Super resolution . microscopy. NSOM. Lecture 18:. High speed microscopy, Part 2. High speed microscopy, Part . 2: Spatial . light modulator microscope and other 3D sensors. Making laser scanning confocal microscopes faster. Resonant scanner confocal. Binding Activated Localisation . Microscopy. Abhik Bose, 154033002, PhD 1. St. Year. . Under the . Guidence. of . Prof.. . Arindam. Chowdhury. Chemistry, IIT Bombay. CHS 802. Slides: http://abhik.net/presentations.html. Lecture 16:. Super-resolution microscopy: Part . 2. Lecture 16: Super-resolution microscopy and TIRFM. Single molecule imaging. Total internal reflection fluorescence microscopy (TIRFM). Super-resolution techniques . Basic Notion of . English Morphology. Meeting . 2. Presentation Outline. Word vs. lexeme. Word with predictable meaning. Paradigmatic – syntagmatic morphology. walks, walked, walk, walking. sentence: . Jenny . Malmstrom. AFM invented by Binning and co-workers in 1986. . Belongs to the Scanning Probe Microscopy family. Binning et al., Physics Review Letters 1986. AFM invented by Binning and co-workers in 1986. . Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Andres Collazo, Director Biological Imaging Facility. Wan-. Rong. (Sandy) Wong, Graduate Student, TA. Lecture . 7: Confocal Microscopy. Replica of van 1670 Moody Use the information in this tutorial to supplement the visuals in lab and the Chapters 1 8 and 9 in your lab manual Replica of Culpepper tripod microscope built c 1725 by Co Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. Amina Mustafa. Department of Molecular Biology. Virtual University of Pakistan. Invented by :. Antonie. van Leeuwenhoek. (Father of Microbiology). Microscopy. USES:. Diagnostics. Identification. Histology – Tissue analysis. AIM : T THEORY: Microscope is the most commonly used piece of apparatus in the laboratory. It produces greatly enlarged images of minute objects. LIGHT MICROSCOPE A Light Microscope can be simple or ELECTRON. . MICROSC. O. PY. . . . Electron. . micros. c. opy. . (EM). . is a technique. . u. s. ed. . for. . obtaining. . high re. s. olu. t. ion. . images. . of. . biological and non-biologi.