PPT-Principles and Practices of Light Microscopy

II Brightfield optics resolution and aberrations Transillumination Microscope Sample Objective Tube lens Intermediate image plane Projection Eyepiece Object plane Back focal plane pupil. Lecture . 09: . Polarization and DIC. Lecture 9: Polarization and DIC. Review Contrast and Phase Contrast. Polarization. Birefringence. Nomarski. . (Differential . Interference Contrast). Resolution. Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Lecture . 7: Confocal Microscopy. Optical Sectioning: adding the third dimension. Wide-field . Imaging. Point . Spread Function. Deconvolution. Lecture 13:. Super-resolution microscopy: Part I. Lecture 13: . Fluorescent labeling, multi-. sprectral. imaging and FRET. Review of previous lecture. FRET. FLIM. Super resolution . microscopy. NSOM. Lecture 06: . Fluorescence Microscopy. Lecture . 6: Fluorescence . Microscopy. Detectors for Microscopy, Part 2. CMOS, PMT and APD. Phenomenon . of Fluorescence. Energy . Diagram. Rates . of excitation, emission, ISC. Lecture 18:. High speed microscopy, Part 2. High speed microscopy, Part . 2: Spatial . light modulator microscope and other 3D sensors. Making laser scanning confocal microscopes faster. Resonant scanner confocal. Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Andres Collazo, Director Biological Imaging Facility. Wan-. Rong. (Sandy) Wong, Graduate Student, TA. Lecture . 7: Confocal Microscopy. II: . Brightfield. optics, resolution, and aberrations. Trans-illumination Microscope. Sample. Objective. Tube lens. Intermediate image plane. Projection Eyepiece. Object plane. Back focal plane (pupil). Microscopy is the technical field using microscopes to view samples and objects that can not be seen without unaided eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron and scanning probe microscopy.. Replica of van 1670 Moody Use the information in this tutorial to supplement the visuals in lab and the Chapters 1 8 and 9 in your lab manual Replica of Culpepper tripod microscope built c 1725 by Co 1Many different types of light microscopesare available each with their own strengths and limitationsA main tenant of good microscopy is to select the right tool microscope for the job at handPlease c KAARTHIK J. INTRODUCTION. A fluorescence microscope is a optical microscope that uses fluorescence and phosphorescence instead of , or I addition to reflection and absorption to study properties of organic or inorganic substances.. Lecture 06: Fluorescence Microscopy. Andres Collazo, Director Biological Imaging Facility. Ke Ding, Graduate Student, TA. Wan-Rong (Sandy) Wong, Graduate Student, TA. Lecture 6: Fluorescence Microscopy. Amina Mustafa. Department of Molecular Biology. Virtual University of Pakistan. Invented by :. Antonie. van Leeuwenhoek. (Father of Microbiology). Microscopy. USES:. Diagnostics. Identification. Histology – Tissue analysis. AIM : T THEORY: Microscope is the most commonly used piece of apparatus in the laboratory. It produces greatly enlarged images of minute objects. LIGHT MICROSCOPE A Light Microscope can be simple or