PPT-Principles and Practices of Light Microscopy

II Brightfield optics resolution and aberrations Transillumination Microscope Sample Objective Tube lens Intermediate image plane Projection Eyepiece Object plane Back focal plane pupil. Chelsea Aitken. Peter Aspinall. Advantages Over Light Microscopy. Resolution of light microscopy limited by Rayleigh Criterion. If two objects cannot be seen as distinct structures, then they may be considered coincident in space. Lecture 13:. Super-resolution microscopy: Part I. Lecture 13: . Fluorescent labeling, multi-. sprectral. imaging and FRET. Review of previous lecture. FRET. FLIM. Super resolution . microscopy. NSOM. lecture. 16. November 2011. Fourier. plane. Point . object. Image. f. f. f. f. a. a. ’. Magnification. : m=1. Angles. : sin(. a. ’)=sin(. a) .  . a. ’=. a. Magnification and resolution: the Abbe limit. II. MENA3100,OBK, . 29.01.15. We don’t read all. You don’ have to read all. 1.3 Specimen Preparation. Read it. 1.4.1 Bright-Field and Dark-Field. Definetly. 1.4.2 Phase-Contrast. Cursori. 1.4.3 Polarized-Light. Fire Protection Laboratory Methods Day. June 25, 2014. Paul M. Anderson. Graduate Research Assistant. University of Maryland. Department of Fire Protection Engineering. Transmission Electron Microscopy. Jenny . Malmstrom. AFM invented by Binning and co-workers in 1986. . Belongs to the Scanning Probe Microscopy family. Binning et al., Physics Review Letters 1986. AFM invented by Binning and co-workers in 1986. . Applications of Light Microscopy. Observe less detailed features of intact cell than advanced microscopy (i.e. electron and laser). Shape, presence of flagella, diagnostic stain, arrangement of cells, some large internal features. II: . Brightfield. optics, resolution, and aberrations. Trans-illumination Microscope. Sample. Objective. Tube lens. Intermediate image plane. Projection Eyepiece. Object plane. Back focal plane (pupil). (SEM) Electron Microscopy (SEM) and TEM Scanning electron microscopy is used for inspecting topographies of specimens at very high magnifications using a piece of equipment called the scanning electr COLLEGE BOTANY: SEM - VI, PAPER - DSE4T: ANALYTICAL TECHNIQUES IN PLANT SCIENCES, UNIT - 1: FLUORESCENCE MICROSCOPY What is fluorescence microscopy? A fluorescence microscope is an optical microscope Theoretically a microscope is an array of two lenses Need of M icroscopy Living organisms, cells and their organelles which are too small to be seen with the human eye, can only be seen with the aid O'Rourke LG, Pitulle C, Hegarty BC, Kraycirik S, Killary KA, Grosenstein P, et al. Bartonella quintana in Cynomolgus Monkey (Macaca fascicularis). Emerg Infect Dis. 2005;11(12):1931-1934. https://doi.org/10.3201/eid1112.030045. DENTAL COLLEGE, LATUR (MAHARASHTRA). DEPARTMENT OF ORAL PATHOLOGY CONDUCTING ONLINE COURSE. A Webinar on: Hidden eyes IN PATHOLOGY. 1. DEPARTMENT OF ORAL PATHOLOGY AND . MICROBIOLOGY. ELECTRON. . MICROSC. O. PY. . . . Electron. . micros. c. opy. . (EM). . is a technique. . u. s. ed. . for. . obtaining. . high re. s. olu. t. ion. . images. . of. . biological and non-biologi.