Operation Point 20 2volts2 S2 Results of the DEP simulation showing thheight in the chamber The DEP force with a Germinant solution redcolorValve Germinated sproes Germinant solution ID: 205421
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Ungerminated spores, phase-bright Germinated spores, phase-grey to phase-dark (a) (b) S1: Verification of spore germination by validating the loss of refractility of spores using phase contract microscopy. (Magnification: 1000 X, DMLB, Leica Microsystems Inc., Bannockburn, IL.) About 80% of spores have germinated 30 minutes after adding germinant solution, 10mM L-alanine and 2mM inosine. Operation Point: ( 20 ) 2volts2 S2: Results of the DEP simulation showing thheight in the chamber. The DEP force, with (a) Germinant solution (redcolor)Valve Germinated sproes Germinant solution (redcolor)Valve Germinant solution (redcolor)Valve Germinant solution (redcolor)Valve Germinant solution (redcolor)Valve Germinated sproes Germinant solution (red color)Valve S3: Delivery of germinant through the PDMS layer. (a) On-chip ungerminated spores. (Microscope Magnification: 400 X. Axiostar Plus, Carl Zeiss MicroImaging, Inc., Thornwood, NY, Pictures were taken by digital camera with magnification varied with respected to focus.) (b, c) Germinated spores with germinant delivered from valve channels in the third PDMS layer. Phase-dark germinated spores (arrows) can be seen in (b) and (c), particularly near the valve channels.