Analysing 10X Single Cell RNA-Seq Data v2026-02

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Analysing 10X Single Cell RNA-Seq Data v2026-02 Simon Andrews simon.andrewsbabraham.ac.uk Course Outline How 10X single cell RNA-Seq works Evaluating CellRanger QC Exercise Looking at CellRanger QC reports Dimensionality Reduction (PCA,

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01
Analysing 10X Single Cell RNA-Seq Data v2026-02

Simon Andrews
simon.andrews@babraham.ac.uk<br>
02
Course Outline How 10X single cell RNA-Seq works

Evaluating CellRanger QC
[Exercise] Looking at CellRanger QC reports

Dimensionality Reduction (PCA, tSNE, UMAP)
[Exercise] Using the Loupe cell browser

R Frameworks for scRNA analysis
[Exercise] Analysing data in R using Seurat<br>
03
How 10X RNA-Seq Works Cells Barcoded Beads Oil RT Reagents Gel Beads in Emulsion (GEMs)<br>