nano switches to detect novel noncoding RNA expression Scott A Tenenbaum SUNY College of Nanoscale Science and Engineering Nanobioscience The Basic sxRNA Concept OR Structurally interacting RNA ID: 1042781
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1. sxRNA: Using RNA-based, nano-switches to detect novel non-coding RNA expression Scott A. Tenenbaum SUNY- College of Nanoscale Science and Engineering, Nanobioscience
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3. The Basic sxRNA Concept+ORStructurally interacting RNA A 3-way junction structure created from trans-interaction An RNA sequence changes or “switches” the structure of the sxRNA in a designed manner This results in the formation of a new or strengthened stem-loop structure Simple Duplex FormationStructural RNAFormation(sxRNA)RNA-RNA Interactions
4. Structurally Interacting RNA (sxRNA)
5. sxRNA Technology Platform BasicssxRNA molecule has three componentsStem loop – target of proteinFlanks – complementary to trigger RNACoding region – makes desired protein Gene Coding RegionFlanksStem loop3’5’sxRNAs are modular, i.e. change flanks for targets, change gene for use
6. sxRNA and SLBP ControlThe presence of a stem-loop turns on protein production when the sxRNA complex attracts binding proteinHistone stem loop binding proteinTranslationProteinTranslationNo stem loopStem loopGeneGeneX
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10. sxRNA T7 -Transcription
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14. sxRNA Potential Applications Molecular toolStem cell quality controlmiRNA expression in cancerGenetic disordersCystic FibrosisDuchenne muscular dystrophyGlycogen storage diseasesPolycystic kidney diseaseCeliac diseaseCharcot-Marie-Tooth diseaseHemophiliaSickle-cell diseaseTissue/Stem Cell EngineeringKidney repairLiver repairHeartCancerBreastLungColonPancreaticThyroidBladderAntiviral, Vaccine, Imaging
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16. Funding