PPT-Transcriptomes: Microarrays and RNA-seq

Genomics Lesson 81 Hardison 31615 1 transcriptomes 31615 2 Transcriptome All the DNA that is transcribed in all the cells in an organism Proteincoding genes Both primary transcript and mature mRNA. Cancer Institute, Bethesda, Maryland, USA5. and . transcriptomics. Daniel Hurley. What are we going to talk about?. Understanding the core principles and ‘root hypothesis’ of . transcriptomics. Choosing between different . technologies. How to design an experiment. scriptomedataproducedbyTheCancerGenomeAtlas (TCGA)toidentifylarge-scaledifferencesinRNAspli- cingbetweentumorandnormalcontrolsamplesacross 16distinctcancertypes.Whileweobservednoobvious biasesincasset A 100002000030000400005000060000010000200003000040000 B 5000100001500020000250003000035000010000200003000040000 Correlations of hybridisation signals of wild type DNA target in different experiment February 12, 2012 PM1 MicroPlate™ Carbon Sources A1 Negative Control A2 L-Arabinose A3 N-Acetyl-D-Glucosamine A4 D-Saccharic Acid A5 Succinic Acid A6 D-Galactose A7 L-Aspartic Acid A8 L-Proline A With Emphasis on Resolution of the Perloidea. Pickett, BD. Shiozawa, DK. Whiting, MF. Davis, NG. Ridge, PG. Introduction. Data & Objectives. Data. Transcriptomes. 7 Plecoptera . – I’ll show you which ones. RNA-. seq. BMI 877. Spring . 2017. Colin Dewey. colin.dewey@wisc.edu. Overview. Some motivation: axolotl. RNA-. seq. . technology. The . RNA-. seq. . quantification problem. Generative probabilistic models and Expectation-Maximization for the quantification task. Microarrays. Established. in 2006. as a center . supporting. . functional. . genomics. . research. in . nation and . internationwide. Comprehensive and . state. . of. . the . art. . functional. MICRO ARRAY (BIOCHIPS). A . semiconductor . device. It . is used to detect the DNA makeup of a human cell. .. M. icro . arrays contain millions of DNA strands designed to . pair . with their other half as the liquefied human cells are poured over . transcriptomic. ) . data analysis. Ståle. . Nygård. , Bioinformatics core facility, OUS/UiO. staaln@ifi.uio.no. Gene expression. Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product.. Corresponding authorHaben Fesseha, MVSc, DVM Assistant Professor, Department of Veterinary Surgery and Diagnostic Imaging, School of Veterinary Medicine, Wolaita Sodo University, P. O. Box 138, Wolait Barbara Radovani. Genomics. . Master . of. Advanced . genetics. , UAB. Transcriptome. . Source. : https://www.genome.gov/13014330/transcriptome-fact-sheet/. The transcriptome, the entire repertoire of transcripts in. MBI401-High throughput Data analysis. Mamta Sagar. Department of Bioinformatics. UIET-IBSBT, CSJM University, Kanpur. 1. INTRODUCTION. Functional genomics involves the analysis of large datasets of information derived from various biological experiments. One such type of large-scale experiment involves monitoring the expression levels of thousands of genes simultaneously under a particular condition,. Digestive System Tissue Microarrays for Improved Disease Research