Laboratory Demonstration - 1 Analytical Chemistry

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Laboratory Demonstration - 1 Analytical Chemistry Dr Sotiris Kyriakou 5th October 2026 Chromatography is a separation technique based on the different interactions of compounds with two phases, a mobile phase and a stationary phase, as the

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Laboratory Demonstration - 1 Analytical Chemistry Dr Sotiris Kyriakou 5th October 2026<br>
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Chromatography is a separation technique based on the different interactions of compounds with two phases, a mobile phase and a stationary phase, as the compounds travel through a supporting medium.
Mobile Phase A solvent that flows through a supporting medium
Stationary Phase A layer or coating on the supporting medium that interacts with the analytes (example: Silica, Alumina, C18, C4, etc)
Supporting medium A solid surface on which the stationary phase as bound or coated Introduction to chromatography<br>
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Principles of chromatography The main principle involved in column chromatography is the adsorption of the solutes of the solution with the help of a stationary phase and afterward separates the mixture into independent components.

At the point when the mobile phase together with the mixture that requires to be isolated is brought in from the top of the column, the movement of the individual components of the mixture is at various rates.

The components with lower adsorption and affinity to the stationary phase head out quicker when contrasted with the greater adsorption and affinity with the stationary phase. The components that move rapidly are taken out first through the components that move slowly are eluted out last.

The absorption of solute molecules to the column happens reversibly.

The pace of the movement of the components is communicated as: Rf (retardation factor )= the distance traveled by solute/ the distance traveled by the solvent<br>