According to the recommendation of theICSH Westergren method is th - PDF document

. According to the recommendation of the(ICSH) Westergren method is the method of choice for ESR. And subsequently introduced techniques fordisadvantages which limit its application. Therefore newer INTRODUCTION:evaluate the prognosis and diagnosis of certain diseases. ItAlthough it is non-specific, but this inexpensive test iscommonly used in the diagnosis and monitoring of certain Hina Mushtaq, Tariq Mehmood, Imran Khan, Nabila Razvi Hina Mushtaq,Department of Haematology PNS Shifa, Karachi. Tariq Mehmood, Page-110 JBUMDC 2019; 9(2):109-112 methods but they show difference outcomes. Some concludedthat there is agreement between two methods. While othershow significant difference between the readings. Thethe westergen method for dertermination of ESR inMETHODOLOGY:Total 300 blood samples from the patients referred atthe inclusion criteria, were selected by Non-Probability,Consecutive Sampling, included in the study. Sample sizewas calculated according to WHO sample size calculator x (SD of difference)For calculation of sample size, Correlation coefficient wasCorrelation coefficient, confidence interval, values of MeanBoth male and female patients of all age groups sufferingFever, malaise, cough, muscular, joint pain or generalizedincluded in the study.the study. Furthermore, on visual inspection samples withfrom the study.hospital to conduct the study. Written consent was takenand procedure of the study. 3ml blood sample was obtainedby venipuncture into Tri potassium Ethylene diamine tetra-EDTA) vacuum tubes. The samples wereWestergren method performed according to ICSH’swas transferred manually from an EDTA tube into ESRvacuum tubes containing sodium citrate. The sedimentationof the RBC’s was recorded through visual determination,after 1 hour using a timer. Automated ESR was performed by TEST1 automated ESRanalyzer (ALIFAX SpA, Italy). This analyzer functions onanalysis. It uses K3-EDTA or K2-EDTA as an an

ticoagulant.the confounding factors like temperature of lab, effect ofdesigned for this study.Pearson correlation coefficient analysis has been used toWestergren method. Data has been stratified for age, gender,symptoms and duration of symptoms to address effectmodifiers. Post stratification Correlation coefficient hasRESULTS:The mean age of the patients was 40.58±20.13 years. Therewere 188 (62.7%) male and 112 (37.3%) female patients inthe study group. Among the causes for measurement ofarthralgia (15.3%). The duration of symptoms ranged fromin Table 1.The ESR measured by Westergren method ranged from 2 hour. to 131 mm/1 hour. With a mean of 39.94±29.60 hour, while ESR measured by automated method hour. to 120 mm/1 hour. With the hour. There was significantlystrong correlation between automated and the Westergrenshown in Table 2. Similar results were observed across allage, gender and type of symptoms groups as shown in Tablesout the effects of confounding factors like variations inof various conditions that affect plasma proteins and the Hina Mushtaq, Tariq Mehmood, Imran Khan, Nabila Razvi 111 JBUMDC 2019; 9(2):109-112 Age GroupsGeneralized Weakness 112 (37.3%) Table 1: Baseline Characteristics of Study Subjects Correlation Westergren29.11±27.12 Automated 112 Age Gender ESR(MEAN±SD) Table 2: Correlation between Automated and Westergren ESR P=() 43.55±34.11 Correlation Westergren 113 YesYesYesYesYesYes Weakness Symptom Table 3: Correlation between Automated and Westergren ESR ** Correlation is significant at the 0.01 level (2-tailed) In developed part of the world, the traditional Westergrenlimited. To resolve this issue, several techniques to introduceof the Westergren method with diluted or undiluted sampleswhile others use very new technologies. The modificationincludes the use of undiluted EDTA samples, easier use,such as Hepatitis B and HIV is increasing, their transmissionby blood is a cardinal hazard

for medical staff. It is thereforeimperative to ensure the safety of laboratory staff againstthese infectious diseases. Automated techniques offer moreposition of tube, and diluents’ ratio) on the final reading.Moreover, in a specified time the number of evaluatedagainst the standard Westergren method.automated and the Westergren method for determination ofESR (r=0.945; p= )The ESR measuredby Westergren method ranged from 2 mm/1 hr. to 131 hour. Whilehr. to 120 mm/1 hour, with a mean of 41.67±32.43 mm/1hour.the two methods. Asif et al. (2012) reported significantstrong correlation between Westergren and automatedgren and automatedDrashti in 201610, Kamal in 201811, Cerutti et al. in 2011 (r=0.816, p)Wiwanitkit in 2001 (r=0.98; p)VenapusaWestergren method are correlated at 95%. But, simultaneously Correlation Between Automated And The Westergren Method For Determination Of ESR systemic bias, but failed to determine its nature. Al fadhliESR readings; While, readings for normal and slightly raised. This study has fewlimitations: as, it was performed on TEST1 automated ESRanalyzer (ALIFAX SpA, Italy), that functions on the principleauto analyzers that function on different principles.were included so it was difficult to comment on thereliable and can be used for routine estimation of ESR. Todetermine more reliability on the automated analyzer, furthercorrelation between the Westergren method and automatedDanesh J, Wheeler JG, Hirschfield GM. C-reactive proteinWu LA, Tan SL, Wright RS, Kopecky SL, Burrit MF, Santrachrate in patients with unstable angina. Arch Pathol Lab MedArikan S, Akalin N. Comparison of the erythrocytesedimentation rate measured by the Micro Test 1 sedimentationanalyzer and the conventional Westergren method'Ann SaudiOzdem S, Akbas HS, Donmez L, Gultekin M. Comparisonof TEST 1 with SRS 100 and ICSH reference method for theAtas A, Cakmak A, Soran M, Karazeybek H. Comparativestudy between the Ves-M

atic and Micro erythrocytesedimentation rate method. J Clin Lab Anal 2008;22(1):70-Ibramanian A, Rangarajan K, Pandey RM, Gandhi JS, SharmaV, Bhoi SK. Evaluation of an mated erythrocyte sedimentationrate analyzer as compared to the Westergren manual methodPathol Microbiol 2011;54(l):70-4. Ajubi NE, Bakker AJ, van den Berg G. Determination of thelength of sedimentation reaction in blood using the Test 1Mahlangu JN, Davids M. Three-way comparison of methodsClin Lab Anal 2008;22(5):346-52.Asif N, Uppal R, Mehmood S, Ahmed A, Ali S, Afzal M.Validation of automated ESR methods with conventionalDrashti NS, Shaila NS.comparitive study of Westergrenmethod and Automated ESR analyser(Microsed-10). JMSCR11.Kamal P,Vyankatesh TA, Shilpa S.Evaluation of micro ESRmethod with Westergren methodfor determination ofHashemi R, Majidi A, Motamed H, Amini A, Najari F,Tabatabaey A. Erythrocyte Sedimentation Rate MeasurementUsing as a Rapid Alternative to the Westergren Method. Emerg(Tehran) 2015;3(2):50-3.13Sönmez Ç, Guntas G, Kaymak AÖ, Akkaya N, Akýn KO.Test-1 and Automatic Westergren Device with ReferenceWestergren Method. Gazi Med J 2014;25(2):52-4Cerutti H, Muzzi C, Leoncini R, Scapellato C, Cortelazzo A,by VES Matic Cube 80 in relation to inflammation plasmaproteins. J Clin Lab Anal 2011;25(3):198-202.Wiwanitkit V. Comparative study between the WestergrenHorsti J, Rontu R, Collings A. Comparison between theStaRRsed Auto-Compact Erythrocyte Sedimentation RateInstrument and the Westergren Method. J Clin Med ResVennapusa B, De La Cruz L, Shah H, Michalski V, ZhangQY. Erythrocyte sedimentation rate (esr) measured by thestreck esr-auto plus is higher than with the sediplast westergrenmethod. Am J Clin Pathol 2011;135(3);386-90.AlFadhli SM, Al-Awadhi AM. Comparison of erythrocyteand conventional Westergren method using the Bland and2003;141(2):115-6. Page-112 JBUMDC 2019; 9(2):109-112 Hina Mushtaq, Tariq Mehmood, Imran Khan, Nabila Ra