PPT-Epigenomics & Transcriptomics

Studies in MESA Yongmei Liu MD PhD FAHA Wake Forest School of Medicine Funded through NIH Roadmap Epigenomics Program and NHLBI At MESA Exam 5 20092011 2500 participants. Exploring transcription factor binding and the . epigenomic. landscape. Saurabh. Sinha. (several slides here are courtesy of . Lisa . Stubbs). Eukaryotic genomes . are. complex structures comprised of modified and unmodified DNA, RNA and many types of interacting proteins. Nature vs. Nurture. Once nurture seemed clearly distinct from nature. Now it appears that our diets and lifestyles can change the expression of our genes. How? By influencing a network of chemical switches within our cells collectively known as the . Genomics. 2015/16. Silvia del Burgo. Introduction. Same genome for all cells that arise from single fertilized egg, Identity? .  . Epigenomic. signatures . Epigenomics: study of chemical changes in DNA and histones (without altering DNA sequence) that are inheritable and involved in regulating gene expression, development, tissue differentiation and suppression of TE. . Ivana de la Serna. Block Health Science 388. 383-4111. ivana.delaserna@utoledo.edu. Outline. 1. . Epigenetics. -definition and overview. 2. DNA methylation/. hydroxymethylation. 3. . Histone. modifications. Jim Noonan. GENE 760. Transcriptomics. Introduction to . RNA. -. seq. m. RNA-. seq. . Martin and Wang . Nat Rev Genet . 12:671 (2011) . Wang . et al. . . Nat Rev Genet . 10:57 (2009) . Poly-A subtraction. UNIT . 5. Gene expression – A misnomer ?. In reality, gene expression can only be quantified by looking at protein products in the cell (. via. proteomic approaches).. T. he . term has been co-opted to describe differences in transcript (mRNA) levels.. Transcriptomics – towards RNASeq Federico M. Giorgi – federico.giorgi@gmail.com Analisi del Genoma e Bioinformatica Corso di Laurea Specialistica in Biotecnologie delle piante U. sing . Partek . Flow . Software. Mar 27, 2019. Ansuman Chattopadhyay, PhD. Asst Director, Molecular Biology information service. Health sciences library system. University of pittsburgh. ansuman@pitt.edu. Microarray . Analysis and Omics Technology. Introduction. Approximately humans have 25000 genes. Only . a fraction of these are actively expressed as mRNAs at any one time. . Hybridization between the cDNA reverse transcribed from a biological sample to a pre-designed complementary DNA probe arranged on a slide, or array, is the basis of DNA microarrays.. Microarray . Analysis and Omics Technology. Introduction. Approximately humans have 25000 genes. Only . a fraction of these are actively expressed as mRNAs at any one time. . Hybridization between the cDNA reverse transcribed from a biological sample to a pre-designed complementary DNA probe arranged on a slide, or array, is the basis of DNA microarrays.. Heads of Medicines Agencies www.hma.eu An agency of the European Union European Medicines Agency www.ema.europa.eu GenomicsGenetics, Transcriptomicsand EpigeneticsubgroupreportDidier Meulendijks, Raneesh Ramarapu. Mentor: Dr. Sara Thomasy. Comparative Ophthalmology and Vision Sciences Laboratory (COVSL). Corneal Endothelium. Hexagonal single cell layer . Non-proliferative in most species . Active cells that constantly pump out water – maintain corneal transparency . grown in the presence of . silver. , . copper. , . gallium . and . gold. metal salts at sublethal concentrations: similarities and differences at the transcriptome level. . Daniel A. Salazar . Alem. We developed a topic . modeling method . to learn nested tree-structured gene expression modules (GEMs) that reflect cellular functions and transcriptomic processes of a specific cell type in the tumor microenvironment of colorectal cancer. .