PPT-Kinetics of DNA Damage and Repair in Fish using the Zebrafish

Danio rerio as a model Chris A McCabe 1 Chris W Theodorakis 2 Theodore B Henry 1 and Mark G J Hartl 1 Introduction Environmental stressors can damage the DNA of aquatic organisms. . zebrafish. model for inflammatory bowel disease (IBD): preliminary results. Simon Haarder. 1, 3. , Per Kania. 1. , Poul Hyttel. 2. , Thomas . Lindebo. Holm. 3. and Kurt Buchmann. 1. 1 . MUTAGENESIS. DNA DAMAGE. DNA REPAIR. RECOMBINATION. MUTAGENESIS. Mutations. Mutations are heritable permanent changes in the base sequence of DNA.. Point mutations may be . transitions. (e.g. GC→AT) or . cellular sensing/responding, . and repair. Rebecca Fry, Ph.D.. DNA damage. DNA damage, due to environmental factors and normal metabolic processes inside the cell, occurs at a rate of 1,000 to 1,000,000 molecular lesions per cell per day. . DNA damage and repair and their role in carcinogenesis. A DNA sequence can be changed by copying errors introduced by DNA polymerase during replication and by environmental agents such as chemical mutagens or radiation. (. Danio. . rerio. ) . as a model. Chris A. . McCabe. 1. , Chris W. Theodorakis. 2. , Theodore B. Henry. 1. and Mark G. J. . Hartl. 1. Introduction . Environmental stressors can damage the DNA of aquatic organisms. By: Laura Pung, Trina Morgan, Mark & Mimi Magdolen. Who Are We?. Laura Pung, Claims Manager . Wheaton|Bekins. Trina Morgan, National Account Adjuster. Wheaton|Bekins. Mark Magdolen, Repair Firm. Weathersby Guild, San Antonio-Austin. Hello scientists. ,. Welcome to . BioEYES. ! We are bringing you an exciting experiment. For the next week your goal will be to learn all you can about . zebrafish. and their similarities to humans. You will work with the . and repair. Rebecca Fry, Ph.D.. DNA damage. DNA damage, due to environmental factors and normal metabolic processes inside the cell, occurs at a rate of 1,000 to 1,000,000 molecular lesions per cell per day. . Mary Mitchell, JC Achenbach, Sean . Tibbetts. , Lee Ellis. What are Zebrafish?. 2. Danio . rerio. – part of the minnow family. Small tropical freshwater fish. Native to South Asia. ~ 1.5 – 2 inches. Principles of Molecular Biology. Group Members. Maira Aleem. Bilal Naveed. Tanzeela Raza. Maheen. Malik. Zaigham. Abbas. DNA Structure. DNA = Deoxyribose nucleic . acid.. Four Nucleotides . - A. denine. A one-step DNA repair process involving an enzyme that reverts the DNA lesion to the original nucleotides. Examples are 0 6 -methylguanine DNA methyltransferase (MGMT) andphotolyases. MGMT Topic 3. DNA REPAIR. . . . S.O ANADOZIE (Ph.D.). DNA DAMAGE . DNA is the repository of genetic information in each living cell, the cellular DNA must be replicated (duplicated), maintained, and pass down to the daughter cells accuracy. . Elise Whitlinger. 1,2. , Madison Rager. 2. , Andrew Kwok-Ping Lam. 2. , Hui Feng. 2. Freeport Area High School, 625 S Pike Road, Sarver, PA 16055. 1. , Boston University School of Medicine, 72 East Concord Street, K-712A Boston, MA 021182. REPLICATION ERRRORS AND THEIR REPAIR. The nature of mutations. Transitions. Transversions. Some replication errors escape proofreading. Mismatch repair removes errors that escape proofreading. Structure of .