PPT-Lecture 5: Mutation and DNA Repair

Chapter 12 Presented by Dr Laurie M Erickson Chair of the Department of Health Sciences Blitstein Institute of Hebrew Theological College Chicago IL USA 121122 Mutations Mutation any heritable change in the genetic material. Enter CPR® – Cell Phone Repair Franchise Systems, Inc. (CPR®) – an independent service organization dedicated to the on-premises repair of cell phones, iPods, and electronic devices at all of our locations from Yukon, OK, to Los Angeles, CA. Davis Repair has built its reputation by providing friendly, honest and reliable automotive services within the greater Denver area. The Brown family, which has operated the business since 2008, includes Davis Repair owners Larry and Ellen and service writers Josh and Hannah. Reliable Centennial auto repair. Davis Automotive Service and Repair provides expert service for all makes and models of motor vehicles. We are a family owned and operated company offering complete range of auto repair services in the Greenwood Village, Englewood, Centennial and South Denver metro area. Davis Repair has built its reputation by providing friendly, honest and reliable automotive services within the greater Denver area. The Brown family, which has operated the business since 2008, includes Davis Repair owners Larry and Ellen and service writers Josh and Hannah. Reliable Centennial auto repair. Davis Automotive Service and Repair provides expert service for all makes and models of motor vehicles. We are a family owned and operated company offering complete range of auto repair services in the Greenwood Village, Englewood, Centennial and South Denver metro area. MUTAGENESIS. DNA DAMAGE. DNA REPAIR. RECOMBINATION. MUTAGENESIS. Mutations. Mutations are heritable permanent changes in the base sequence of DNA.. Point mutations may be . transitions. (e.g. GC→AT) or . A. frameshift. B. missense. C. neutral. D. silent. E. transition. REEF Question. A normal 90 . bp. length of DNA gives rise to a 30 amino acid polypeptide after transcription and translation. An A. G. Mechanisms for the repair of damaged DNA are probably universal. . Escherichia coli . possess 3 different mechanisms for the repair of DNA.. DNA REPAIR MECHANISMS. Excision repair. Photoreactivation. Updated Summer 2015. by J. D. Hendrix. Mutations: Definitions. Lethal . mutation: results in death of the cell, and therefore cannot be propagated or studied. Conditional mutation: One that is expressed only under certain environmental conditions; for example, a temperature-sensitive mutation. Program Overview . Discussion Outline. DNA Repair. ABCs of DNA Repair. DNA Repair Defects in Cancer. DNA Repair Defects Can Be an Achilles' Heel. Synthetic Lethality and Cancer DNA Repair Defects. Increased Sensitivity of . Principles of Molecular Biology. Group Members. Maira Aleem. Bilal Naveed. Tanzeela Raza. Maheen. Malik. Zaigham. Abbas. DNA Structure. DNA = Deoxyribose nucleic . acid.. Four Nucleotides . - A. denine. DNA damage DNA gets damaged a lot ! DNA damage DNA gets damaged a lot! >200,000 events/human cell/day DNA damage Occurs 2 ways 1) spontaneously 2) mutagens : damage DNA Transposons Discovered by Barbara McClintock The types of proteins an organism possesses depend upon the . sequence of nucleotides. The two main types of gene mutations include:. Point mutation: one base pair is replaced for another base pair.. If we have an error one in a million this mean we will have 3000 errors during each replication cycle of the genome.. The genome of a typical mammalian cell . accumulates many . thousands of lesions during a 24-hour period.. . REPLICATION – GENERAL PRINCIPLES. START. Must be ready . Must know where to start . FINISH. Must all finish . Must ensure that each piece of DNA is replicated only once . Therefore must know where to finish . ABSTRACT Chemotherapy often targets dividing cells by causing DNA damage that leads to replication-dependent toxic lesions. Cells possess several overlapping DNA damage repair pathways that allow them