PPT-BS2009 - GENOMES DNA replication and repair

REPLICATION GENERAL PRINCIPLES START Must be ready Must know where to start FINISH Must all finish Must ensure that each piece of DNA is replicated only once Therefore must know where to finish . Andrey Grigoriev. Director, Center for Computational and Integrative Biology. . Rutgers University. 2. What are we going to do?. Observe effects of fundamental processes. Estimate their relative contribution. GO TO . BIOINTERACTIVE WEBSITE. DNA replication questions. Why is DNA replication so important?. In what part of the cell cycle does DNA replication occur?. What enzyme brings in the new DNA nucleotides that form complimentary pairs with the old DNA strand?. WHAT YOU WILL LEARN. -process of DNA making copies . -role of enzymes in copying DNA. -leading and lagging strands in DNA replication. READING Q. ’. s . 1-LIST:. -the 3 phases of semi-conservative replication. 20.1 part 2. DNA replication. Earlier on in this unit we learned about mitosis or cell division.. In order for mitosis to occur and chromosomes to duplicate themselves, the DNA must copy itself exactly.. DNA damage and repair and their role in carcinogenesis. A DNA sequence can be changed by copying errors introduced by DNA polymerase during replication and by environmental agents such as chemical mutagens or radiation. Semiconservative vs. Conservative. DNA Polymerase. RNA Primase. Okazaki Fragments. Replication Errors. TeachWithFergy . Preview File. . Please . enjoy this preview of your Power Point. . Some slides appear blank because they have been removed.. Dr. Kevin Ahern. Structure. Structure. Structure. One Strand of DNA. Structure. Introduction. Structure. Introduction. DNA Structure. DNA Forms. Semi-Conservative Replication. Discontinuous. Replication. What you need to know:. The major goals of the Human Genome Project.. How . prokaryotic genomes compare to eukaryotic genomes. .. Applications of bioinformatics to medicine, evolution, and health.. The activity and role of transposable elements and . . §. 4.3a DNA Replisome. . §. 4.3b DNA Damage. §. 4.3c DNA . Repair. IV. NUCLEAR BIOCHEMISTRY. §. 4.3a . DNA Replisome. Synopsis . 4.3a. DNA replication involves making . copies or “. replicas” of DNA. . Understanding DNA structure and replication is a prerequisite for understanding/using the principal tools of molecular biology . R. R. 0. R0. R0. 0. R0. R0. R. 0. R. RR. R0. 0. R0. 00. DNA: Structure & Replication. Directions: Front of card write term. On back write definition and draw picture . Chromosomes. Thread-like structure made of coiled DNA. Gene. Segment of DNA that codes for a specific trait. Nucleotide. Something to think about... When a species . reproduces. , there is . genetic continuity . maintained from one generation to the next.. WHAT . is responsible for this continuity?. HOW . does this continuity happen?. In . their 1953 announcement of a double helix structure for DNA, Watson and Crick stated, . "It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.". ABSTRACT Chemotherapy often targets dividing cells by causing DNA damage that leads to replication-dependent toxic lesions. Cells possess several overlapping DNA damage repair pathways that allow them