PPT-Kinetics of DNA Damage and Repair in Fish using the Zebrafi

Danio rerio as a model Chris A McCabe 1 Chris W Theodorakis 2 Theodore B Henry 1 and Mark G J Hartl 1 Introduction Environmental stressors can damage the DNA of aquatic organisms. Enter CPR® – Cell Phone Repair Franchise Systems, Inc. (CPR®) – an independent service organization dedicated to the on-premises repair of cell phones, iPods, and electronic devices at all of our locations from Yukon, OK, to Los Angeles, CA. Nucleotide Excision Repair XP and CS. BiS222. By . Jooyoung. Chang . 장주영. Class #01 / Stu. #20091259. Assembling a Car. An analogy. Steps in building a CAR. Steps in building a . PROTEIN. Q: Most . Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. and repair. Rebecca Fry, Ph.D.. DNA damage. DNA damage, due to environmental factors and normal metabolic processes inside the cell, occurs at a rate of 1,000 to 1,000,000 molecular lesions per cell per day. . Principles of Molecular Biology. Group Members. Maira Aleem. Bilal Naveed. Tanzeela Raza. Maheen. Malik. Zaigham. Abbas. DNA Structure. DNA = Deoxyribose nucleic . acid.. Four Nucleotides . - A. denine. DNA damage DNA gets damaged a lot ! DNA damage DNA gets damaged a lot! >200,000 events/human cell/day DNA damage Occurs 2 ways 1) spontaneously 2) mutagens : damage DNA Transposons Discovered by Barbara McClintock  . Author: Michael Fenech. Affiliation: Genome Health Foundation, North Brighton, SA, 5048, Australia. Email: . mf.ghf@outlook.com. . Introduction. Life as we know it depends entirely on the capacity of cells to utilize energy and molecules in the environment for cellular function and reproduction. . If we have an error one in a million this mean we will have 3000 errors during each replication cycle of the genome.. The genome of a typical mammalian cell . accumulates many . thousands of lesions during a 24-hour period.. . REPLICATION – GENERAL PRINCIPLES. START. Must be ready . Must know where to start . FINISH. Must all finish . Must ensure that each piece of DNA is replicated only once . Therefore must know where to finish . of intense freckling and high melanoma incidence, related to UV sensitivity. The syndrome actually results from defects in any of nine genes involved in repair of UV damage. C. EPAIRING DAMAGED OR ABSTRACT Chemotherapy often targets dividing cells by causing DNA damage that leads to replication-dependent toxic lesions. Cells possess several overlapping DNA damage repair pathways that allow them A one-step DNA repair process involving an enzyme that reverts the DNA lesion to the original nucleotides. Examples are 0 6 -methylguanine DNA methyltransferase (MGMT) andphotolyases. MGMT Topic 3. DNA REPAIR. . . . S.O ANADOZIE (Ph.D.). DNA DAMAGE . DNA is the repository of genetic information in each living cell, the cellular DNA must be replicated (duplicated), maintained, and pass down to the daughter cells accuracy. . REPLICATION ERRRORS AND THEIR REPAIR. The nature of mutations. Transitions. Transversions. Some replication errors escape proofreading. Mismatch repair removes errors that escape proofreading. Structure of .