DNA Cloning 3 Introduction into host cell Restriction Enzymes PCR Polymerase Chain Reaction Antibiotic Selection Antibiotics such as penicillin and ampicillin kill bacteria Plasmids can carry genes that give host bacterium a resistance against antibiotics ID: 932038
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Slide1
Biochemistry
Lecture 3
DNA Technologies
Slide2Slide3DNA Cloning
3
Introduction
into
host cell
Slide4Slide5Slide6Restriction Enzymes
Slide7Slide8Slide9PCR
Polymerase
Chain
Reaction
Slide10Slide11Antibiotic Selection
Antibiotics, such as penicillin and ampicillin, kill bacteria
Plasmids can carry genes that give host bacterium a resistance against antibiotics
Allows growth (selection) of bacteria that have taken up the plasmid
Slide12Expression of Cloned Genes
Slide13DNA Electrophoresis
Slide14Sanger DNA
Sequencing
14
Slide15DNA Sequencing
Slide16Automated DNA Sequencing
16
Slide17Shotgun Sequencing
Slide18Applications of Sequencing
Solving crimes
Identifying genetic diseases
Huntington’s disease
Breast cancer
Sickle cell anemiaV
iral infectionsHuman migration patternsParentage/pedigree/genealogy
18
Slide19Genome Editing:
CRISPR-Cas9
System
19
Doudna
, JA,
Charpentier
E. Science. 2014; 346(6213).
Slide20Electrochemical Sequencing
http://www.youtube.com/watch?v=yVf2295JqUg
Slide21Slide22