PPT-Methylation for Clinical Diagnosis

Garrett Jenkinson PhD Data Scientist Assistant Professor of Biomedical Informatics Division of Computational Biology Department of Quantitative Health Sciences Genetics versus Epigenetics Which is more different at cellular level phenotypically. Lamarck Revisited. Lamarck was incorrect in thinking that the inheritance of acquired characters is the main mechanism of evolution. However, we now know that the inheritance of acquired characters . :. DNA . methylation. II. DNA . methylation. in plants (Ch9). Differences between plants and animals. Auxotroph . vs. . heterotroph. Sessile . vs. . mobile. Motionless and rigid . vs. . migrating and flexible cells. :. DNA . methylation. I. Requirements for epigenetic materials. Need to be . transmitted faithfully . during mitosis and meiosis (possibly along with DNA). -> . cell need to have . maintenance mechanisms similar to semi-conservative mechanism of DNA replication. dim . mutants: DNA Methylation in . Neurospora. . c. rassa. Calvin Summers. Mentor: Andy . Klocko. Selker. Lab. Agenda. :. What is methylation?. How is it controlled?. Why is it important. ?. What is . epigenome with integrative “omics” data analysis.  . Hehuang. “David” . Xie. Sept, 2014. Presentation Outline. Introduction to Epigenetic codes. Genome-wide Hairpin Bisulfite Sequencing. Application on . Daniel Messinger. , Ph.D.. Messinger. Hmmm…. ‘Mothers . who were . pregnant during a 1944 famine in the . Netherlands. were . more likely to have children—and grandchildren—prone to heart disease, diabetes, and obesity. . Ivana de la Serna. Block Health Science 388. 383-4111. ivana.delaserna@utoledo.edu. Outline. 1. . Epigenetics. -definition and overview. 2. DNA methylation/. hydroxymethylation. 3. . Histone. modifications. Detection Methods. Daniel . Goan. Anna Tseng. Joanna . Tychowski. Feb 2, 2012. MSP. Overview. DNA Digestion Based. COBRA. Real-time MSP. MethyLight. Bisulfite Sequencing. Pyrosequencing. MassARRAY. Southern-blot hybridization. Fade Gong & Nam Nguyen. Introduction. DNA . methylation. in mammals occurs at . CpG. sites, where it serves many functions:. Self vs. non-self identification (such as in viral/bacterial infection). . . Global and . G. enome-Wide Studies. . . Yanbin Dong MD PhD. . Regents’ . Professor, Pediatrics. Georgia Prevention Institute. Department of Pediatrics. Medical College of Georgia. Stephen Clark – . Reik. Lab, . Babraham. Institute. Background. Bulk analyses mask intercellular differences . vs.. Background. Bulk analyses mask intercellular differences . Silva . J, Smith . A, . 110 Report Figure 1: Methylenetetrahydrofolate reductase role in generegulation and methionine- homocysteine metabolism Gynecology Obstetrics & Reproductive Medicine 2016;22(2): A 38 year-old pregna . WS 2019/20 - lecture 15. 1. Bioinformatics III. Epigenetics. . refers. . to. . alternate. . phenotypic. . states. . that. . are. . not . based. on . differences. in . genotype. .. They. . I. Requirements for epigenetic materials. Need to be . transmitted faithfully . during mitosis and meiosis (possibly along with DNA). -> . cell need to have . maintenance mechanisms similar to semi-conservative mechanism of DNA replication.