BeomKang Huh Sarah Czaplewski Client Dr Wan Ju Li Advisor Professor Block Cartilage bioreactor Overview Joint Trauma and Degeneration Current Treatments Future Directions NonDestructive Testing for Tissue Engineering ID: 930391
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Slide1
Sarah SpringbornLuisa MeyerBeomKang HuhSarah Czaplewski
Client: Dr. Wan Ju LiAdvisor: Professor Block
Cartilage bioreactor
Slide2OverviewJoint Trauma and DegenerationCurrent TreatmentsFuture DirectionsNon-Destructive Testing for Tissue Engineering Requirements for High Field MR MR Compatible Bioreactor DesignsAssessment of Materials and Deformation Methods
Bioreactor Construction and Validation
Slide3Cartilage Damage and TreatmentsCartilage cannot repair itselfAccident/InjuryOsteoarthritisCartilage between joints wears away
Affects 21 million AmericansCurrent TreatmentsCartilage transferJoint replacement
Slide4Cartilage Regeneration
Goal: repair lesions to articular
cartilage
Stem cells removed from patient
Cells grow in scaffold
Cells mature and differentiate due to applied conditions
Mature cells are implanted to fill lesions
Slide5Non-Destructive TestingCurrent MethodsTesting throughout stages of developmentDestroys cellsIsolates proteins, genes, etc for analysis
Proposed MethodTesting throughout stages of developmentCells remain viableImages of mechanical loading
Slide6Material
BiocompatibleAutoclave and MR safePhysical Design3 inch diameterLeak-proof removable capMechanical LoadingTissue cannot move5-20% Deformation
~3 mm
~1.5 cm
Requirements for MR and Compression
Slide7Plunger Compression
Side View of Bioreactor
Manual twist or push compression
Removable fixation
Cap
Guide rails for fixation to slide into
Compressor
Tissue
Legs to lift tissue off the bottom
Side View of Fixation
Rubber Seal
Slide8Lever Compression
Slide9Angled Compression
Slide10Design Matrix - MaterialsResistance
to Heat*Transparency*DurabilityCostEase of FabricationTotal(35)Bioceramics10
2
1
1
1
15
Teflon
10
2
5
2
2
21
PVC
4
10
4
3526Poly-carbonate
61045
530
* The score is weighted by a factor of two
Slide11Design Matrix – Overall DesignsRisk of Leakage*
Fixation*Maintain Force*DurabilityEase of FabricationCostTotal (50)Plunger
8
6
8
4
5
3
38
Lever
6
8
4
3
2
1
28
Angled
6864
4335
* The score is weighted by a factor of two
Slide12Proposed Design
Manual twist or push compression
Removable fixation
Cap
Guide rails for fixation to slide into
Compressor
Tissue
Legs to lift tissue off the bottom
Side View of Fixation
Side View of Bioreactor
Rubber Seal
Clear Polycarbonate Plastic
Slide13Bioreactor ValidationCreate “mock-up” of designDetermine thermal conductivity of imaging coil
Observe temperature decayEstablish necessary volume of medium for imagingAir space
Slide14Bioreactor ConstructionOrder componentsPolycarbonateNon-ferrous hardwareFabricate prototype
Slide15ReferencesArticular Cartilage Restoration." 01 Feb 2009. American Academy of Orthopaedic Surgeons, Web. 11 Oct 2009. <http://orthoinfo.aaos.org/topic.cfm?topic=A00422>."Osteoarthritis."
MedicineNet. Web. 11 Oct 2009. <http://www.medicinenet.com/osteoarthritis/article.htm>. Tuli, Richard, Wan-Ju Li, and Rocky Tuan. "Current State of Cartilage Tissue Engineering." Arthritis Research & Therapy 5.5 (2003): n. pag. Web. 11 Oct 2009. <http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=193737>.
Slide16AcknowledgmentsThank you to:Dr. LiProfessor Block
Slide17Questions?