PPT-(3) Gene Expression

C Transcription A What is Gene Expression B Structure and Functions of RNA Gene Expression E Splicing and posttranslational modification D Translation Gene Expression C State where transcription occurs. Inducible gene expression. kinetics of . β-galactosidase. enzyme induction. Add inducer. start transcription = mRNA accumulation. mRNA translation = protein accumulation. Remove inducer. Stop. transcription (. limma. package. 20 March 2012. Functional Genomics. Linear regression. Fit a straight line through a set of points such that the distance from the points to the line is minimized. The slope of the line is adjusted to minimize the . Understanding:. Gene expression is regulated by proteins that bind to specific base sequences in DNA . The environment of a cell and of an organism has an impact on gene expression. Nucleosomes help to regulate transcription in eukaryotes. CONSTITUTIVE GENE PRODUCTS ARE NEEDED BY THE BODY AT ALL TIMES. TUMOR SUPRESSOR PROTEINS. ATP. ENZYMES THAT CONTROL CELLULAR RESPIRATION. ADAPTIVE GENE PRODUCTS ARE ONLY REQUIRED UNDER CERTAIN CONDITIONS. What does the operon model attempt to explain?. the coordinated control of gene expression . in bacteria. bacterial resistance to antibiotics. how genes move between homologous regions of DNA. the mechanism of viral attachment to a host cell. Stephen Montgomery. smontgom@stanford.edu. montgomerylab.stanford.edu. @. sbmontgom. Identifying the genes and variants responsible for disease.. Monogenic to polygenic diseases. Rare to common variants. . Differential . expression, clustering, networks, and functional enrichment. STEMREM 201 Fall 2012. Aaron . Newman, Ph.D.. 10/17/12. A . genomics approach . to . biology involves…. A plethora of . University of Notre Dame. Kristin Hager, Associate Teaching Professor, Biological Sciences. Joseph O’Tousa, Professor, Biological Sciences. WH Freeman. Marc Mazzoni, Senior Editor, Life Sciences. Elaine Palucki, Editor, Adjunct Assistant Professor Biology, Brooklyn College. Draw 8 boxes on your paper. Gene regulation accounts for some of the phenotypic differences between organisms with similar genes.. 2005-2006. Gene regulation in bacteria. Control of gene expression enables individual bacteria to adjust their metabolism to environmental change. Controlling gene expression is often accomplished by controlling transcription initiation.. Regulatory proteins . bind to DNA to either block or stimulate transcription, depending on how they interact with RNA polymerase.. Drosophila. Embryos using . lacZ. Transgenes. June 18. th. ABLE 2014. University of Oregon, Eugene. Cathy Silver Key. Julie Gates. Jessica Sawyer. Kirsten . Guss. Acknowledgements. Funding from . Roberta . analysis - outliers. V2: batch effects. V3: data imputation. What . is measured by microarrays?. Microarray normalization. Differential gene expression (DE) analysis based on microarray data. Detection of outliers. We performed a genome-wide analysis of gene expressionto identify germline- and sex-regulated genes.Using mutants that cause defects in germ cell proliferationor gametogenesis, we identiÞed sets of g Venet. , et al.. PLoS. Computational Biology, 2011. Molly Carroll. Biomedical Research Methods. Characterize mechanism in the model. Derive a marker that changes when the mechanism is altered. Show .