PPT-Sequence and Structure Analysis of Surface Glycoprotein of SARS-CoV-2 from India Variants

COVID19 is a pandemic across the world It is caused by a novel coronavirus severe acute respiratory syndrome coronavirus 2 SARSCoV2 The SurfaceSpike Glycoprotein of SARSCoV2 which plays a key role in the receptor recognition and cell membrane fusion process is composed of two subunits S1 and S2 In the present work we have searched for SurfaceSpike glycoprotein in the NCBI protein database and origin from India the search hit out 192 protein sequences as on 20 June 2020 Further the sequences were aligned using SurfaceSpike glycoprotein from WuhanChina Origin and on the basis of the sequence length of 1273 the sequences were screened Out of 192 input protein sequences 177 sequences were complete in the length of 1273 aminoacids Comparing all the sequences via sequence alignment mode in MEGAX exhibited a complex diversified outcome and reported 32 sequences The protein sequence id QKI286851 was identified as a root and 31 protein sequences as a mutantvariant QKI286851 was subjected to 3D protein structure modelling As no fulllength structural template was identified in the database Automated homology modelling SwissModel server and threading based ITasser were considered for the structure determination SwissModel reported a partial structure from amino acid length 27 to 1146 A fulllength structure is obtained from the ITasser server The structures were analyzed using the ProSA and Ramachandran plot 31 identified mutations were manually incorporated in the protein structure and a total of 31 mutants were created Further these proteins are in a process to study and understand the structural changes and their impact on the proteinprotein interaction and proteindrug interaction. Andrew Morris. Advanced Topics in GWAS. Toronto, 30 May 2012. Introduction. GWAS have been successful in detecting . novel loci for complex traits:. typically characterised by common variants of modest effect;. Gustavo Caetano - . Anolles. 1. PowerPoint by Casey . Hanson. Protein Sequence, Structure, and Function | Gustavo Caetano - Anolles | 2015. Exercise. In this exercise we will be doing the following:. BIOST 2055. 04/06/2015. Last Lecture . Genome-wide association study has identified thousands of disease-associated loci. Large consortium performs meta-analysis to further increase the sample size (power) to detect additional loci. Features: . (. i. ) Provides standardised ‘. DeepSEA. score’ for noncoding variants. (ii) Provides info on chromatin feature(s) and cell type(s) to concentrate on. (iii) Identify base-resolution sequence features by . 1 1. You have published many papers on coronaviruses and even warned of the possibility of a serious spillover event from animals to humans. Do you feel like your warnings were not appropriately hee full-length genome sequencing of SL-CoV Rp3 (primer sequences will be provided upon request). These primers were then used to obtsamples (Rf1, Rm1, Rp1 and Rp2). PCR productswith E.Z.N.A Gel Extractio NTo whom correspondence should be addressed E-mail zlshiwhiovcn ZS zhangsyiozaccn SZ linfawangcsiroau L-FW Severe acute respiratory syndrome SARS emerged in 2002-3 in southern China The origin of its An Initiative of DBT-AIs. Coordinated by . National Institute of Biomedical Genomics (NIBMG), . Kalyani. . P. articipating Institutes:. Teams:. . . NIBMG:. . Arindam. . Maitra. , . Nidhan. Biswas, . Section 1: Introduction and biological databases.. Section 2: Sequence alignment.. Section 3: Gene and promoter prediction.. Section 4: Molecular phylogenetics.. Section 5: Structural Bioinformatics. Central dogma of molecular biology. Term is due to Francis Crick. The conversion DNA . → protein is not direct, RNA is involved. DNA is the information store, RNA is messenger (mRNA), transporter (tRNA), biomolecular nanomachine (rRNA). eucaryotic glycoprotein tein has layer of of X- ray diffraction diffraction However, chemical analysis of the halobacterial surface layer as well as comparison with surface layers of other archaebacte Li N, Xiao L, Cama VA, Ortega Y, Gilman RH, Guo M, et al. Genetic Recombination and Cryptosporidium hominis Virulent Subtype IbA10G2. Emerg Infect Dis. 2013;19(10):1573-1582. https://doi.org/10.3201/eid1910.121361. 1. The Gutell . Lab @ The . University of Texas at . Austin. Major Topics. Importance of RNA in the Cell. Major Changes in Paradigms. . Grand Challenges in Biology. Identification and Characterization of RNA Structure. 11% of the edited variants were insertions and 4% were deletions.. RESULTS. Chromosome 29 was used to compare 1000 Bull Genomes Project run7 to local AGIL data.. 1000 Bull Genomes Project run 7 identified 149,684 variants on chromosome 29.