PDF-Proc.Nat.Acad.Sci.USAVol.68,No.9,pp.2135-2140,September1971Theoretical

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ProcNatAcadSciUSA681971TorpracticalpurposesitisconvenienttousethenormalizedintensitydistributionfunctionIebiNhv4EEPavlOavIRabebvN126EaVbVhvVIVfobpNa4tt0DbEEiPavIP4avbvNI

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Proc.Nat.Acad.Sci.USAVol.68,No.9,pp.2135-2140,September1971Theoretical: Transcript

ProcNatAcadSciUSA681971TorpracticalpurposesitisconvenienttousethenormalizedintensitydistributionfunctionIebiNhv4EEPavlOavIRabebvN126EaVbVhvVIVfobpNa4tt0DbEEiPavIP4avbvNI. Proc.Natl.Acad.Sci.USA87(1990)wereprepared(25),andtheDNAinsertwasexcisedwithEcoRI,isolatedbylowmeltagarosegelelectrophoresis,andrandom-primedforexpressionanalysis.Theexpressionlev-elsandsizesofthemRNA Proc.Natl.Acad.Sci.USA73(1976)29290.6OD0.50.41+mPMU0.3LDEPTH(mm)IIIIIIII00.51.0FIG.1.Atracedcomputeroutputdisplayingtheaveragedopticaldensitiesoflayersinthespecimenofcerebralcortexshownbelow.Thescanni Proc.Natl.Acad.Sci.USA93(1996)Table1.PNDsdefinedbyautoimmuneantibodiesNeurologicsyndromeParaneoplasticnamePhenotypeTumorAntibodyCerebellardegenerationPan-cerebellardysfunction(Purkinjecell)TypicalBrea 612PanofskyProc.Nat.Acad.Sci.USA70(1973)FRASCATIwX.I,0.01(CERN31)1976(NAL)1972ft((SLAC-RLA))lANL-0.1975BNL-GCERN-PSLAANL-ZGSMoscowCORNELL0(TSUKUBA)MOSCOWUNIMRODV19740DESYBEVATRON*CEAYEREVAN0NINACOSMOT 868CellBiology:OsbornandWeberFIGS.1-7.(Legendappearsatbottomofthefollowingpage.)Proc.Nat.Acad.Sci.USA73(1976) Proc.Nat.Acad.Sci.USA73(1976)869FIG.8.Recoveryofcytoplasmicmicrotubulesin3T3cellsafterColc Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl 1134CellBiology:Halpernetal.Proc.Nat.Acad.Si.USA71(1974)x900Co4-1U.N.600zin00:300_05010150TIME(hours)FIG.1.GrowthofW-256(ratbreastcarcinosarcoma)cellsinculture.Walker-256cellsweregrownintwomodifiedMcC I,fromFlavobacteriumokeanokoites,isamemberoftheunusual,typeIIsclassofrestrictionendonucleasesthatrec-ognizeaspecificDNAsequenceandcleavenonspecificallyashortdistanceawayfromthatsequence(1).Ibindstheco Proc.Natl.Acad.Sci.USA90(1993)pHP45QC(13)bySmaIdigestionandgelpurification.pIB415isaderivativeofpIB410(ofIncPreplicon)containingvirAA63/240,avirAderivativelacking178aminoacidsintheperiplasmicdomain(14 Genetics:Slupskaetal.Table1.ComparisonofmutAandmutCwithmutA,CStrainLac+revertantsper(CC105)108cells,no.+2.3 Proc.Natl.Acad.Sci.USA88(1991)5593Table1.YeaststrainsNuclearMitochondrialStraingenotypegenotypeRef.947aade2ura3-52p09ID41-6aadellysi15161p+15C1036mit-;COXJintron116splicing-defectivepointmutant(trans- Cellsrespondtodevelopmentalcuesandenvironmentalstimulibyalteringgeneexpression.Often,transmissionofdifferentupstreamsignalsinvolvescommoncomponentsyetelicitsdistinct(butappropriate)outcomes.Numerousag Proc.Natl.Acad.Sci.USA91(1994)9991pooled;theproteinswereprecipitatedwith(NH4)2SO4anddesaltedasdescribedabove,exceptthattheproteinswereelutedinavolumeof3.5mlusingabuffercontaining50mMNaCland50mMTris-HC Proc.Natl.Acad.Sci.USA82(1985)ofcellsoveriodinevapors.Cellsthataccumulateglycogenstainbrown,whereasthosethatdon'tremainyellow.Quan-titativetrehaloseandglycogendeterminationsweremadeaccordingtoLilliean

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