PPT-VBC-605 Unit II Enzyme kinetics

1 Enzyme concentration 2 Temperature 3 Hydrogen ion concentration or pH 4 Substrate concentration 5 Inhibitors 6 Product concentration 7 Activators 8 Physical agents Factor affecting enzyme kinetics. C483 Spring 2013. Questions. 1. Enzymes . that join two substrates and require energy of a nucleoside triphosphate (such as ATP) to do so are called. A. ) . isomerases. .. B. ) . lyases. .. C. ) ligases.. Lecture 8: Tuesday, 9/15/15. Today’s Objective. Kinetics of rectilinear motion. . What is Kinetics?. Kinetics is the study of the relations between unbalanced forces and the resulting changes in . (CRE) is the field that studies the rates and mechanisms of chemical reactions and the design of the reactors in which they take place.. Lecture. 15. Lecture . 15 . – . Tuesday 3/12/2013. Enzymatic Reactions. ZONE40 ZONE47 ZONE46 ZONE48 ZONE54 ZONE55 ZONE49 ZONE44 ZONE43 ZONE42 ZONE41 ZONE38 ZONE39 ZONE45 ZONE46 ZONE52 ZONE53 603 601 601 601 601 601 601 605 605 607 607 607 608 608 760 TX1 700 761 606 606 6 Kinetics Purpose: 1) Investigate the kinetics of LDH purified from bovine heart and muscle 2) Learn how to determine kinetic information 3) Understand the effects of inhibitors on enzyme activity En a Virtual Biologics Clinic. Dr Ben Parker PhD, MRCP. Consultant Rheumatologist, Manchester Royal Infirmary. Honorary Senior Lecturer, University of Manchester. To cover. Why the need for innovation in biologics services?. Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. MAT 493. 5/6/2015. Outline. Introduction. Homogeneous Case. Non-Homogeneous Case. Semi-Linear Case. Introduction. What is a Chemical Reaction?. A process that transforms one or more substances into another.. Topic 2: . Enzyme. . Kinetics. What is an Enzyme?.  . Enzymes: .  are biological molecules (proteins) that act as . catalysts*.  and help complex reactions . take place. Basically, they . break a protein down into its amino . . Day . 5: Begin Kinetics Lab. Warm Up. If you are given . 15 M . stock solution and you need to make . ONLY 20 mL . of a . 6 M solution. , how much of the stock solution should you use?. Agenda. Demonstration. Citric Acid cycle or Tricarboxylic Acid cycle or Krebs Cycle. Overview and brief history. Pyruvate Dehydrogenase Complex (PDC) and its control. Reactions of TCA cycle or CAC. Amphibolic nature of TCA cycle. Inhibition-. The decrease in enzyme activity/loss of activity. exert effect by decreasing . affinity of the enzyme for the substrate. decreasing the amount of active enzyme available for catalysis. by a combination of these effects. Lipitor: HMG-CoA reductase, inhibits a liver enzyme that is important in biosynthesis of cholesterol (>$100 billion total sales since 1996).. Viread & Emtriva: reverse transcriptase inhibitors, anti-retrovirus (HIV)..