PPT-VBC-605 Unit II Enzyme kinetics

1 Enzyme concentration 2 Temperature 3 Hydrogen ion concentration or pH 4 Substrate concentration 5 Inhibitors 6 Product concentration 7 Activators 8 Physical agents Factor affecting enzyme kinetics. 1 In this exercise we will look at the catalytic behavior of enzymes. You will use Excel to answer the questions in the exercise section. At the end of this session, you must hand in answers to all C483 Spring 2013. Questions. 1. Enzymes . that join two substrates and require energy of a nucleoside triphosphate (such as ATP) to do so are called. A. ) . isomerases. .. B. ) . lyases. .. C. ) ligases.. (CRE) is the field that studies the rates and mechanisms of chemical reactions and the design of the reactors in which they take place.. Lecture. 15. Lecture . 15 . – . Tuesday 3/12/2013. Enzymatic Reactions. Kinetics Purpose: 1) Investigate the kinetics of LDH purified from bovine heart and muscle 2) Learn how to determine kinetic information 3) Understand the effects of inhibitors on enzyme activity En Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. MAT 493. 5/6/2015. Outline. Introduction. Homogeneous Case. Non-Homogeneous Case. Semi-Linear Case. Introduction. What is a Chemical Reaction?. A process that transforms one or more substances into another.. E S <=> ES <=> ES* <=> EP <=> E P. Michaelis-Menten Kinetics. E S <=> ES <=> ES* <=> EP <=> E P. Rate of Formation. Substrate. Product. Enzyme. Enzyme-. Topic 2: . Enzyme. . Kinetics. What is an Enzyme?.  . Enzymes: .  are biological molecules (proteins) that act as . catalysts*.  and help complex reactions . take place. Basically, they . break a protein down into its amino . . Day . 5: Begin Kinetics Lab. Warm Up. If you are given . 15 M . stock solution and you need to make . ONLY 20 mL . of a . 6 M solution. , how much of the stock solution should you use?. Agenda. Demonstration. Christian Kendall and Galen Mack-Crane. Reactions. Mass-Action Kinetics. Michaelis and Menten (1913). . First Order. Time (years). [C] conc. by %mass. Second Order Unimolecular. Enzyme-Substrate Reactions. Lipitor: HMG-CoA reductase, inhibits a liver enzyme that is important in biosynthesis of cholesterol (>$100 billion total sales since 1996).. Viread & Emtriva: reverse transcriptase inhibitors, anti-retrovirus (HIV).. 1 In this exercise we will look at the catalytic behavior of enzymes. You will use Excel to answer the questions in the exercise section. At the end of this session, you must hand in answers to all ODE. Stochastic. Units. With concentrations* (ODE simulations only):. concentrations (. eg. moles/liter) for species. Unit concentration / unit time for reactions. With particles (ODE or stochastic):.