PPT-Figure 5 Figure 5. . Histopathologic analysis (hematoxylin and eosin stain) of tissues

Feng J Hu Y Ma Z Yu Q Zhao J Liu X et al Virulent Avian Infectious Bronchitis Virus Peoples Republic of China Emerg Infect Dis 2012181219942001 httpsdoiorg103201eid1812120552. Objective: to understand how blood stains can give forensic scientists information about a crime.. blood. Blood is what moves oxygen around our body from the lungs to the organs that need oxygen and then back the lungs for more oxygen.. HAEMATOXYLIN & EOSIN. . Supervised by : . Dr.Sherifa. . Prepared by: Reem Aldossari. Lama . Alabdi. H&E staining:. The best known and most widely used staining procedure, . hematoxylin. -eosin staining (H&E), uses . organs. Literally, histology means tissue or web. To study tissues, certain techniques an instruments are used to study tissues. Tissue Preparation. For conventional bright field light microscopy, we follow the following steps:. COMPONENTS 100ml. MODIFIED MAYER Xiaowei Su, PGY-3 & Dr. Geoffrey Murdoch. Adapted from slides by . jen. . nichols. and . robyn. massa. Bacterial Meningitis. Meningitis is an inflammatory disease of the leptomeninges. Abnormal number of white blood cells in the CSF. methylene blue and eosin stain the tissue perfectly if placed in aqueous or alcoholic solutions . This known (. Direct staining . ). Haematoxylin required additional substance known as a mordant . This is (. are held together at their centromeres binds specific proteins, which in turn make up a disk-like structure called the kinetochore. The kinetochore is an attachment site for spindle fibers, which pla Rahimi P, Sohrabi A, Ashrafihelan J, Edalat R, Alamdari M, Masoudi M, et al. Emergence of African Swine Fever Virus, Northwestern Iran. Emerg Infect Dis. 2010;16(12):1946-1948. https://doi.org/10.3201/eid1612.100378. Coscolla M, Lewin A, Metzger S, Maetz-Rennsing K, Calvignac-Spencer S, Nitsche A, et al. Novel Mycobacterium tuberculosis Complex Isolate from a Wild Chimpanzee. Emerg Infect Dis. 2013;19(6):969-976. https://doi.org/10.3201/eid1906.121012. Girouard G, Garceau R, Thibault L, Oussedik Y, Bastien N, Li Y. Adenovirus Serotype 14 Infection, New Brunswick, Canada, 2011. Emerg Infect Dis. 2013;19(1):119-122. https://doi.org/10.3201/eid1901.120423. Song D, Kang B, Lee C, Saif LJ, Ha G, Kang D, et al. Transmission of Avian Influenza Virus (H3N2) to Dogs. Emerg Infect Dis. 2008;14(5):741-746. https://doi.org/10.3201/eid1405.071471. refractile. body within the vegetative cells. Procedure: (fig.10) . 1- Prepare a fixed smear.. 2- Flood the smear with 5% malachite green and let the stain react for 1 minute .Using your . bunsen. burner, heat the top of the stained slide until steam rises but do not let it boil. This step is performed by passing the burner back and over the slide for about 5 minutes.. Brown JD, Stallknecht DE, Beck JR, Suarez DL, Swayne DE. Susceptibility of North American Ducks and Gulls to H5N1 Highly Pathogenic Avian Influenza Viruses. Emerg Infect Dis. 2006;12(11):1663-1670. https://doi.org/10.3201/eid1211.060652. Hung T, Neafie RC, Mackenzie I. Baylisascaris procyonis Infection in Elderly Person, British Columbia, Canada. Emerg Infect Dis. 2012;18(2):341-342. https://doi.org/10.3201/eid1802.111046.