PPT-Electron Microscopy Lab X-ray microanalysis in the electron microscope

Tutor Peter Harris Click on the buttons to move between slides Make sure you are in Slide Show mode The aims of this course are to explain how Xrays are generated in the electron microscope. Jim Atherton. Development of Light Microscope. 1590 Hans Zacharias Janssen. 1660 Robert Hook. Onserved. . cells (cork). 1. Klein, Aaron E. The Electron Microscope: A Tool of Discovery. New . Y. ork,McGRaw. Chelsea Aitken. Peter Aspinall. http://www.zyvexlabs.com/EIPBNuG/EIPBN%20images/05Ion.jpg. Intro to Electron Microscopy. Similar to optical microscopy except with electrons rather than photons. Used to image samples with a resolution of 10 Å. Microscopy is the technical field using microscopes to view samples and objects that can not be seen without unaided eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron and scanning probe microscopy.. Replica of van 1670 Moody Use the information in this tutorial to supplement the visuals in lab and the Chapters 1 8 and 9 in your lab manual Replica of Culpepper tripod microscope built c 1725 by Co instrument that produces . enlarged . image of . object. Used to study organisms, cells, and cell parts. Increase image . of object . and . S. how object’s details. MAGNIFICATION. : . increase . of . Peter Harris. Transmission . electron . microscopy. ► What . is transmission electron microscopy?. . ► TEM in biological science . ► CfAM’s TEMs. ► TEM in physical . science . ► Recent developments in TEM. Electron . Microscopic techniques . (EM). 2020-2021. Electron microscopy (EM) is an electron beam which is focused into a small probe across the surface of . a specimen. . The. . first. . electromagnetic lens was developed in 1926 by Hans Busch. Electron microscope follows the same principle of compound microscope, but uses electrons beam as an illumination source instead of light. . Scanning electron microscope (SEM. ). 2- Transmission Electron Microscope. New Techniques in Microscopy:. Confocal Microscopy (Confocal Scanning Laser Microscope). Light Microscope. Electron Microscope. . Electron microscopy (EM) . is an electron beam which is focused into a small probe across the surface of a specimen . . The first electromagnetic lens was developed in 1926 by . Hans Busch. .. . AIM : T THEORY: Microscope is the most commonly used piece of apparatus in the laboratory. It produces greatly enlarged images of minute objects. LIGHT MICROSCOPE A Light Microscope can be simple or (SEM) Electron Microscopy (SEM) and TEM Scanning electron microscopy is used for inspecting topographies of specimens at very high magnifications using a piece of equipment called the scanning electr Theoretically a microscope is an array of two lenses Need of M icroscopy Living organisms, cells and their organelles which are too small to be seen with the human eye, can only be seen with the aid A . fluorescence microscope.  is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to . study . organic . or inorganic substances. The aims of this course are:. ● . to introduce the principles of scanning electron microscopy. ● . to describe the components of the microscope and explain how they work. ● . to highlight some of the problems which can arise during imaging.