instrument that produces enlarged image of object Used to study organisms cells and cell parts Increase image of object and S how objects details MAGNIFICATION increase of ID: 911389
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Slide1
Microscopy
Slide2Microscope
DEFINE: instrument that produces enlarged image of
objectUsed to study organisms, cells, and cell partsIncrease image of object and
S
how object’s details
MAGNIFICATION: increase of object’s apparent sizeRESOLUTION: power to show object’s details clearly
Slide3Light Microscopes
COMPOUND LIGHT MICROSCOPE (LM)To see small organisms and cells
Specimen must be cut thin enough so light can pass through and mounted onto glass slideDifferent set magnifications
Slide4Light Microscope
Slide5Parts of LM
STAGE: supports specimen
LIGHT SOURCE: mirror, light bulb; directs light upwards, through specimenOBJECTIVE LENS: enlarges image of objectNOSEPIECE: holds objective lens of different magnifications
OCULAR LENS
: near eyepiece;
magnifies image furtherCONDENSER/ DIAPHRAGM: controls the amount of light projected to specimen COARSE FOCUS: focuses object into viewFINE FOCUS
: focuses fine details of object into view
Slide6Electron Microscope
At about 2000x magnification images become blurryUses
beam of electrons instead of light to enlarge image of specimenTypes:Transmission Electron Microscope (TEM)Scanning Electron Microscope (SEM)
Black Walnut Tree Leaf
Slide7Transmission Electron Microscope
Transmits beam of electron through very thin slice of specimen
Magnetic lenses enlarge image and project it in screen or photographic platePRO: 200,000x magnificationCON: Can NOT view living things
Slide8Scanning Electron Microscope
Provides 3-D imagesSpecimen not sliced but coated with a fine metal spray
Electrons bounce off metal coating and projected onto fluorescent screen of photographic platePRO: 100,000x magnification; 3-D imageCON: Can NOT be live specimens
Slide9LM vs TEM vs SEM
Mitochondria, mammalian lung
Fly Head
Diatom, 1000x
Slide10USING THE COMPOUND LIGHT MICROSCOPE
WET MOUNT REVIEWMOVING THE SLIDELETTER INVERSIONDEPTH OF FIELDMAGNIFICATION
EYEPIECE (10x) x LENS (10X LOW OR 40X HIGH)=100X, 400X
Slide11USING THE COMPOUND LIGHT MICROSCOPEMEASUREMENT IN MICROMETERS
( µ ) 1mm = 1000 µm and .001mm = 1 µmDIAMETER OF FIELD EXAMPLE UNDER HIGH POWER DIAMETER (LOW POWER)= 4 mm
DIAMETER (HIGH POWER) 40/10 = 4 4000/4 = 1000 µmPROPER CARE AND USAGE
Slide12MICROSCOPE LAB DIRECTIONS SUMMARYTEXTBOOK PAGES 691-694
NOTES: SEE MATERIAL LIST ON P.691SKIP STEP #’S 19-24REVIEW MICROSCOPE DIAGRAM ON THE
TOP LEFT OF P.692 FOR PARTS A AND BYOU DO NOT HAVE TO DO TABLE PC.1FOR PART C, MAKE WET MOUNT SLIDES OF THE LETTERS O, C, AND E AND DRAW WHAT YOU OBSERVE UNDER LOW OR HIGH POWER (10X OR 40X). KEEP YOUR WET MOUNT OF THE LETTER “E” FOR #33.NOTICE WHAT HAPPENS TO YOUR SPECIMEN WHEN YOU
MOVE THE SLIDE
ONE WAY OR THE OTHER.
PAY ATTENTION TO WHAT HAPPENS TO YOUR FIELD OF VIEW WHEN YOU INCREASE MAGNIFICATION (AS WELL AS IF YOU HAVE TO ADJUST YOUR LIGHT DIAPHRAGM).FOR PART D, USE THE COLORED THREADS SLIDE IN THE BACK OF THE ROOM FOR #25-30.FOR PART E, USE THE TRANSPARENT RULER IN THE BACK OF THE ROOM TO MEASURE YOUR FIELD OF VIEW USING THE 4X OBJECTIVE LENS (USE mm MARKINGS AND PLACE THE RULER ON THE FAR LEFT OF YOUR FIELD OF VIEW, MAKE YOUR MEASUREMENT AND THEN CONVERT IT TO MICROMETERS).FINISH THE LAB BY DOING ANALYSIS QUESTIONS #1-6 ON P.694.
Slide13How to Use the Microscope
Slide14MICROSCOPE QUIZ FORMAT21 M.C. QUESTIONS
3 M.C. BONUS QUESTIONS (1 PT. EACH)REVIEW: OUTLINE SHEET, LAB ANALYSIS QUESTIONS, STUDY GUIDE QUESTIONS, MICROSCOPE DIAGRAM p.692