PDF-Ann.N.Y.Acad.Sci.ISSN0077-8923

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ANNALSOFTHENEWYORKACADEMYOFSCIENCES Issue DNAHabitatsandTheirRNAInhabitants Prefaceto DNAHabitatsandTheirRNAInhabitants WhenIturnedmyfocusfromdebatesonthephilosophyofsciencetothephilosophyofbiologyan

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Ann.N.Y.Acad.Sci.ISSN0077-8923: Transcript

ANNALSOFTHENEWYORKACADEMYOFSCIENCES Issue DNAHabitatsandTheirRNAInhabitants Prefaceto DNAHabitatsandTheirRNAInhabitants WhenIturnedmyfocusfromdebatesonthephilosophyofsciencetothephilosophyofbiologyan. ANNALSOFTHENEWYORKACADEMYOFSCIENCES Issue: BlavatnikAwardsforYoungScientists Platetectonicsandplanetaryhabitability:currentstatus andfuturechallenges JunKorenaga DepartmentofGeologyandGeophysics,YaleU Proc.Natl.Acad.Sci.USA73(1976)29290.6OD0.50.41+mPMU0.3LDEPTH(mm)IIIIIIII00.51.0FIG.1.Atracedcomputeroutputdisplayingtheaveragedopticaldensitiesoflayersinthespecimenofcerebralcortexshownbelow.Thescanni etal. Figure1.Thearti Proc.Nat.Acad.Sci.USA91(1994)9761thepGEM4vector(Promega)forsequencing.Specifically,a1.2-kbPstIfragment,whichhybridizedtothemousecDNAprobe,butnottotalmouseDNA,wassubclonedtogeneratethesequencehomologou MedicalSciences:Cherif-Zaharetal.Proc.Natl.Acad.Sci.USA87(1990)6245aatcccggcctgcacagagacggacacaggATGAGCTCTAAGTACCCGCGGTCTGTCCGG60MetSerSerLysTyrProArgSerValArg9CGCTGCCTGCCCCTCTGGGCCCTAACACTGGAAGCAGCTC Proc.Natl.Acad.Sci.USA93(1996)Table1.PNDsdefinedbyautoimmuneantibodiesNeurologicsyndromeParaneoplasticnamePhenotypeTumorAntibodyCerebellardegenerationPan-cerebellardysfunction(Purkinjecell)TypicalBrea Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl ANNALSOFTHENEWYORKACADEMYOFSCIENCES Issue:Annals Reports Targetingepigeneticregulatorsforcancertherapy SusanWee, 1 DashDhanak, 2 HaitaoLi, 3 ScottA.Armstrong, 4 RobertA.Copeland, 5 RobertSims, 6 Steph Dew&CabezaRelationshipbetweenexplicitandimplicitmemoryTable1.Thetraditionalmodel,highlightingthecriticalfeaturesusedtodichotomizeexplicitandimplicitformsofmemory Memoryphenomenon FeatureImplicitExplic Proc.Nat.Acad.Sci.USA70(1978)624~~~~~~~~~~~~24*0aS5!!402040608000020-tO60eo0as--40506~~~~~~~~~~~~~0GROWTHINTHEL00H000)CRW'0NTE0~Z04-20FIG.2.(A)Kineticsofinductionofthechloroplastisoleucyl-tRNAuponexpo Proc.Natl.Acad.Sci.USA93(1996)11529BothLandL*canbeprotonatedonLys-156,butthepKaforprotonationisconsiderablylowerforL*thanforL;pKL*iswhilepKLis10.OneimportanttenetofthismodelisthattheIndsmutationsincre I,fromFlavobacteriumokeanokoites,isamemberoftheunusual,typeIIsclassofrestrictionendonucleasesthatrec-ognizeaspecificDNAsequenceandcleavenonspecificallyashortdistanceawayfromthatsequence(1).Ibindstheco Proc.Natl.Acad.Sci.USA82(1985)6415fragmentwasisolatedfroma0.8%agarosegelbyusingtheapparatusforpreparativegelelectrophoresis.DNASequencing.DNAsequencingusingdideoxynucleo-tideswasperformedasdescribedby Cellsrespondtodevelopmentalcuesandenvironmentalstimulibyalteringgeneexpression.Often,transmissionofdifferentupstreamsignalsinvolvescommoncomponentsyetelicitsdistinct(butappropriate)outcomes.Numerousag

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