Jankowski Lab AnesthesiaPain Management Jordan Lamb Project Description Goal Better understand molecular mechanisms that lead to muscle pain after injury in order to determine better treatments for musculoskeletal pain ID: 288036
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No Pain, No Gain: Understanding Muscle Pain
Jankowski Lab – Anesthesia/Pain ManagementJordan LambSlide2
Project Description
Goal – Better understand molecular mechanisms that lead to muscle pain after injury in order to determine better treatments for musculoskeletal pain Muscle-specific injury model in miceBlock blood flow in right forepaw by tying off brachial artery (Ischemia)Remove blockage after several hours to allow blood flow again (reperfusion)
Causes changes in the muscle tissue and in gene expression in afferent neurons that carry pain input from muscle to CNSSlide3
Project Description Cont.
Three types of procedures to study pain produced after injuryBehavior – guarding, grip strength, threshold they respond to mechanical stimuliElectrophysiological recording – responsiveness to mechanical, thermal, and chemical muscle stimulationMolecular Assays
My role
Focus on molecular assays
Real-time PCR
analysisSlide4
What? So What? Now What?
Basic laboratory skills – pipetting, weighing chemicals, preparing solutions, mouse handling, etc.Exposed to variety of different proceduresRNA isolation, protein isolation, reverse transcription, PCR, cell cultureAssist with animal surgery About myself
Like working in structured environment – following procedure
Gaining independence
Asking for helpSlide5
What? So What? Now What?
Bettering understanding of neuroscienceClassroom and daily lifeChance to explore other science/medicine related careersPerceptions on research process
A LOT of
work
More respect
Prepared to continue research this summer and in the future