PPT-High Performance Liquid Chromatography (HPLC)

What is HPLC Types of Separations Columns and Stationary Phases Mobile Phases and Their Role in Separations Injection in HPLC Detection in HPLC Variations on Traditional HPLC Ion Chromatography. Supervisor:. Anton Zavialov. By: . Elham Barazeghi. Mehrafarin Ramezani. Affinity Chromatography:. Separation of proteins by reversible interaction of proteins and specific ligand in matrix of column. and Linkage Analysis. Natasha E. Zachara Ph.D.. nzachara@jhmi.edu. What questions might you ask?. What . monosaccharides. modify my protein?. What oligosaccharides modify my protein?. How are these sugars linked to each other?. Announcements I. Exam 2 – Monday. Covering Ch. 6 (topics since exam 1), 7, 8-1, 17, and parts of 22 (up to and including retention and retention factor). Will review topics today. Help Session – Friday 11 to 12, Sequoia 446. Chapter 4. Best Broken into four categories. Theoretical Background. Gas Chromatography. HPLC. Quantitation, Calibration, Standardisation and Validation. Theory. Review of Partitioning. You need to be aware of the following concepts in order to have any idea about this chapter!. Principle and Definition. Principle. The principle of chromatographic separation is very simple. The process is achieved by . distributing the substances. to be separated between a . mobile phase. and a . 7. HPLC Adv. Chrom Revision 1. The P in HPLC is often used as an abbreviation for pressure. Explain why . pump uses high pressure to form liquid through column 2. Draw a block diagram of the components of a typical HPLC. chromatography. . (HPLC). Dr. J. Rajesh. Asso. . Prof. Dept of Chemistry. MSEC, Kilakarai. A. f. orm . o. f. s. e. pa. r. a. t. e. ,. c. o. l. u. m. n . identify,. ch. ro. m. a. to. gr. ap. h. y . ASSISTANT PROFESSOR. PG&RESEARCH DEPARTMENT OF BIOTECHNOLOGY. BONSECOURS COLLEGE FOR WOMEN. THANJAVUR. WHAT IS THE . DOWNSTREAM . PROCESSING. Downstream processing refers to the recovery and purification of biosynthetic products, particularly pharmaceuticals, from natural sources such as animal or plant tissue or fermentation broth, including the recycling of salvageable components and the proper treatment and disposal of waste. It is an essential step in the manufacture of pharmaceuticals such as antibiotics, hormones (e.g. insulin and humans growth hormone), antibodies (e.g. infliximab . #Certification #Dumps #Certification_exam_Dumps
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Certification dumps are collections of questions and answers used to prepare for a certification exam. They are often provided by third-party companies that specialize in exam preparation. Certification dumps are a valuable resource for anyone looking to prepare for a certification exam, as they provide an in-depth overview of the topics and concepts covered on the exam. Additionally, they are often updated with new and relevant information to ensure that the material is as fresh and up-to-date as possible. Certification dumps can save time and money by providing a comprehensive and convenient way to prepare for a certification exam . Botanist. In 1906 Tswett used to chromatography to separate plant pigments. He called the new technique chromatography because the result of the analysis was 'written in color' along the length of the adsorbent column. The word Chromatography is derived fro two Greek . words –. Chroma . means – colour and . graphein. . to write. . Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. Technique for separating the components or solute of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid stream called the mobile phase, and a fixed stationary phase.. Thursday, April 7, 2011. Liquid. . chromatography. What. . is. . chromatography. ?. Derived from the Greek word . Chroma. meaning . colour. , chromatography provides a way to identify unknown compounds and separate mixtures.