PPT-High Performance Liquid Chromatography (HPLC)

What is HPLC Types of Separations Columns and Stationary Phases Mobile Phases and Their Role in Separations Injection in HPLC Detection in HPLC Variations on Traditional HPLC Ion Chromatography. WJEC . IVQ . Science for Work Unit . 3 . WJEC Pathways Science for Work Unit 2.8 . WJEC GCSE Additional Applied Science. Diagram HPLC. solvent. reservoir. pump . detector. waste . injection . port. HPLC. Chromatography (HPLC). Introduction. HPLC . is used to ensure the safety and nutritional quality of . food i.e., chemical . additives . (. i.e., antioxidants such as TBHQ, BHA and BHT), residues . (. Lecture 2. Mahesh Bule. Downstream process . Downstream process . Downstream process . Purification techniques. Filtration/Centrifugation. Precipitation. Liquid-liquid . two-phase separation. Chromatography. BASICS OF CHROMATOGRAPHY Prepared by DR: Sherif Abdel- Naby Abdel- Gawad PhD Analytical Chemistry-Faculty of Pharmacy - Cairo University History of Chromatography Mikhail Tswett is credited with chromatography. . (HPLC). Dr. J. Rajesh. Asso. . Prof. Dept of Chemistry. MSEC, Kilakarai. A. f. orm . o. f. s. e. pa. r. a. t. e. ,. c. o. l. u. m. n . identify,. ch. ro. m. a. to. gr. ap. h. y . . Chemistry. II. Gas. . Chromatography. Mobile . phase. : . inert. . gases. (. helium. , . nitrogen. , argon . etc. ). Stationary. . phases. : in . capillary. . column. Liquid (. siloxane. . polymers. ASSISTANT PROFESSOR. PG&RESEARCH DEPARTMENT OF BIOTECHNOLOGY. BONSECOURS COLLEGE FOR WOMEN. THANJAVUR. WHAT IS THE . DOWNSTREAM . PROCESSING. Downstream processing refers to the recovery and purification of biosynthetic products, particularly pharmaceuticals, from natural sources such as animal or plant tissue or fermentation broth, including the recycling of salvageable components and the proper treatment and disposal of waste. It is an essential step in the manufacture of pharmaceuticals such as antibiotics, hormones (e.g. insulin and humans growth hormone), antibodies (e.g. infliximab . Diese Originalarbeit bietet eine detaillierte Beschreibung elektrochemischer Pulsdetektionsmethoden wie der gepulsten Voltammetrie oder der gepulsten Amperometrie. Die Kopplung solcher Nachweissysteme an ein Stofftrennungssystem (HPLC) erlaubt die Erfassung biologisch aktiver Verbindungen wie Kohlenhydrate, Peptide und Amine in komplex zusammengesetzten Gemischen. Vor- und Nachteile der Methoden werden ebenso diskutiert wie die instrumentelle Ausr?stung und M?glichkeiten der Fehlersuche. (11/97) Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . By . Dr. . Amjad. M. T. Khan. Assistant Professor. Department of Chemistry. AMU, Aligarh. CONTENTS. Introduction to chromatography. History. Principles. Importance. Chromatographic terms. Classification of chromatography. Dr . Debalina. . Basu. Department of Microbiology. Surendranath. College. Paper Chromatography. Principle. What is Descending Paper Chromatography?. Descending paper chromatography is an analytical technique in which the mobile phase moves downward through the stationary phase. In other words, in this method, the development of the paper occurs due to the movement of the solvent downwards on the paper. Therefore, the solvent reservoir should be at the top of the paper. In this process, the movement of the solvent is governed by gravity as well as capillary action.. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. Technique for separating the components or solute of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid stream called the mobile phase, and a fixed stationary phase.. . Chemistry. II. Chromatography. Real . samples. . are. . generally. . complex. . mixtures. . and. . substances. . interfere. . with. . each. . other. in . their. . analyses. . . Chromatography.