PPT-I. General Description of Enzymes

A Chemical composition 1 Enzymes are proteins compounds of high molecular weight containing Carbon C hydrogen H oxygen O sulfur S nitrogen N 2 Possess primary secondary tertiary sometimes quaternary structure. Definition of an enzyme. Enzyme. is . protein . . catalyst. (i.e. . increase the rate of reactions. ). . NOT. changed during the reaction. Reactions. Most reactions take place in a number of steps which need to be carefully controlled if the cell is to function properly. Enzymes are the most important controllers of cellular reactions. Catalysts. Apoenzymes. , . Holoenzyme. , . Abzymes. , . Synzyme. , . Ribozyme. , . Extremozyme. , Historical Development in . Enzymology. , . Characteristics of enzymes, Enzyme Vs Chemical Catalysts. , Enzyme mechanism & Activation Energy, Enzyme Specificity, Fischer and . Enzymes speed up chemical reactions.. Everything that happens in a cell. Growth. Reproduction. Interaction with the environment. Response to stimuli. Is the result of a chemical reaction.. A chemical reaction is a change in the arrangement. 6.4 Cloning & Biotechnology. Learning Objectives. Understand the methods used for immobilising enzymes.. Understand the advantages of using immobilised enzymes.. Enzymes as Catalysts. Enzymes are used to speed up chemical (metabolic) reactions e.g. respiration or photosynthesis- so why use enzymes in industry?. Are specific for . what they . catalyze . and do not permanently change during reactions. End in the word -. ase. Function of Enzymes. Enzymes work by lowering the activation energy (the amount of energy needed to start a reaction). . E S <=> ES <=> ES* <=> EP <=> E P. Michaelis-Menten Kinetics. E S <=> ES <=> ES* <=> EP <=> E P. Rate of Formation. Substrate. Product. Enzyme. Enzyme-. 1. What are enzymes?. Each enzyme has only one specific reaction it can perform. . 2. How many different reactions can a single enzyme perform?. Yes, the same enzyme molecule can be used over and over. Enzyme & Substrate. An enzyme is a globular protein which acts as a biological catalyst by speeding up the rate of chemical reactions. . Enzymes are . NOT. changed or consumed by the reactions they catalyze and thus can be reused. . Enzymes. Back when we discussed proteins, we learned that one critical function of protein was to speed up chemical reactions.. Enzymes. are the proteins that increase the rate of chemical reactions. To the penny placed on your desk.. Observe the penny but don’t touch it. What happens?. Explain why. 2. Give the penny a . little . push. What happened?. Why did the penny move the second time and not the first time?. In order for chemical reactions to take place, . enzymes . must be present to help . speed up . the reaction.. Chemical bonds connect atoms. to make . molecules. .. Chemical . reactions. can do two things:. Presented . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. DNA manipulating enzymes:. Regulation of enzymes Introduction andProperties of Enzymes Enzymes are characteristics to facilitate the Enzyme-catalyzed reactions take place Reactants need to pass over the energy barrier, Catalys