PDF-UnitIII Enzymes

Regulation of enzymes Introduction andProperties of Enzymes Enzymes are characteristics to facilitate the Enzymecatalyzed reactions take place Reactants need to pass over the energy barrier Catalys. Definition of an enzyme. Enzyme. is . protein . . catalyst. (i.e. . increase the rate of reactions. ). . NOT. changed during the reaction. Chapter 2: Section 2.5. 1. Objectives. SWBAT explain the effect of a catalyst on activation energy. . SWBAT describe how enzymes regulate chemical reactions. . and maintain homeostasis.. 2. Starter: How can this be possible. Understanding:. Enzymes have an active site to which specific substrates bind. Enzyme catalysis involves molecular motion and the collision of substrates with the active site. Temperature, pH and substrate concentration affect the rate of activity of enzymes. Presented by Fotios Patsalis . Bio . 446/546 FALL 2016. M/F 13.00-15.50h,. Castetter. . Hall 37. Restriction Enzymes: Molecular Scissors. Restriction enzymes (. endonuleases. ) are specific endonucleases which . Academic . Biology. Enzyme. A large protein molecule. Specific shape with deep folds on its surface. Deep folds form pockets called active site. Enzyme-Substrate. The shape of the . enzyme. . active site . Are specific for . what they . catalyze . and do not permanently change during reactions. End in the word -. ase. Function of Enzymes. Enzymes work by lowering the activation energy (the amount of energy needed to start a reaction). . 1. What are enzymes?. Each enzyme has only one specific reaction it can perform. . 2. How many different reactions can a single enzyme perform?. Yes, the same enzyme molecule can be used over and over. Part 1. . Chapter 3. Learning outcomes. You should be able to:. explain how enzymes work. describe and explain the factors that affect. enzyme activity. use V. max. and K. m. to compare the affinity of different enzymes for their substrates. Food biochemistry and food processing. 2012. Simpson B.K. (Ed.) 2. nd. . edition,. John Wiley & Sons, . Inc.. Enzymes in food processing. Advantages.. Enzymes are proteins with powerful catalytic . Enzymes. Back when we discussed proteins, we learned that one critical function of protein was to speed up chemical reactions.. Enzymes. are the proteins that increase the rate of chemical reactions. To the penny placed on your desk.. Observe the penny but don’t touch it. What happens?. Explain why. 2. Give the penny a . little . push. What happened?. Why did the penny move the second time and not the first time?. Factors affecting enzymatic reactions. Enzymes specificity. Enzymes kinetics and mechanism of action. Control of metabolic pathways. Enzymes inhibition. Enzymes in clinical diagnosis. Enzymes and genetic diseases. Presented . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. DNA manipulating enzymes:. that . catalyze. (. i.e.. , . increase or decrease the rates. of) . chemical reactions. .. . In enzymatic reactions, the . molecules. at the beginning of the process are called . substrates. , and they are converted into different molecules, called the .