PPT-The Transmission Electron Microscope

Bob Ashley 6 21 2013 Overview Systems of the TEM Illumination Condenser lens system Specimen manipulation stage Imaging system Image formation Magnification Recording system Reading List. Transmission Electron Microscope s : 2 TEM/STEMS (80 - 200 kV) SuperSTEM – 2 dedicated aberration corrected STEM s Category: C. Particle Charact erisation in and ex - situ Institute: Univers Jim Atherton. Development of Light Microscope. 1590 Hans Zacharias Janssen. 1660 Robert Hook. Onserved. . cells (cork). 1. Klein, Aaron E. The Electron Microscope: A Tool of Discovery. New . Y. ork,McGRaw. . 2. Compound Light Microscope. Used to observe small objects. Magnifies images up to 2000X their size. 3. Different parts of a microscope. copyright cmassengale. SCI.9-12.B-1.2. - [Indicator] - Use appropriate laboratory apparatuses, technology, and techniques safely and accurately when conducting a scientific investigation. Depth of . f. ield: small. Magnification. Uses lenses arranged in series, made of optical material that bends light. All magnification takes place via lenses. Uses . diascopic. illumination (that is, light is transmitted through the specimen). Magnification up to about 1000x.. See Us at M&M 2020 in Milwaukee instrument that produces . enlarged . image of . object. Used to study organisms, cells, and cell parts. Increase image . of object . and . S. how object’s details. MAGNIFICATION. : . increase . of . Peter Harris. Transmission . electron . microscopy. ► What . is transmission electron microscopy?. . ► TEM in biological science . ► CfAM’s TEMs. ► TEM in physical . science . ► Recent developments in TEM. Scanning electron microscope (SEM. ). 2- Transmission Electron Microscope. New Techniques in Microscopy:. Confocal Microscopy (Confocal Scanning Laser Microscope). Light Microscope. Electron Microscope. . Electron microscopy (EM) . is an electron beam which is focused into a small probe across the surface of a specimen . . The first electromagnetic lens was developed in 1926 by . Hans Busch. .. . (SEM) Electron Microscopy (SEM) and TEM Scanning electron microscopy is used for inspecting topographies of specimens at very high magnifications using a piece of equipment called the scanning electr Theoretically a microscope is an array of two lenses Need of M icroscopy Living organisms, cells and their organelles which are too small to be seen with the human eye, can only be seen with the aid An electron microscope is a microscope that uses a beam of accelerated electrons as a source o f illumination. As the wavelength of an electron can be up to 100,000 times shorter than that of visibl Resolution of SEMTransmissionelectronmicroscopy(TEM)isamicroscopytechniquewherebyabeamofelectronsistransmittedthroughanultrathinspecimen,interactingwiththespecimenasitpassesthrough.Animageisformedfrom To explain the advantages of using an Electron Microscope . To be able to carry out Simple Magnification Calculations. .