with 5 on a a 2 and a and a 20 30 40 50 I I 20 30 40 50 than 005 a 72 had a a x0000 95 homogeneous DiIacLDL cell population showed stimulated proliferation when incubated with GMCSF or M ID: 941859
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interleukin-1, interleukin-2, had no with 5- on a a 2 and a and a 20 30 40 50 I I 20 30 40 50 than 0.05 a 72 had a a � 95% homogeneous DiI
-ac-LDL(+) cell population] showed stimulated proliferation when incubated with GM-CSF or M-CSF (Figs. 4, 5) as found with mixed glial cultures. We
also observed direct mitogenic effects of native and recombinant forms of multi-CSF (Table 1) upon ameboid microglia, as re- ported previously by
our laboratory (Giulian and Baker, 1986) and recently confirmed by Frei and Fontanna (1986). The ac- t 100 150 200 hr with 100 ng 20 30 40 20 30
40 50 had no 100 ng had no and 100 ng but not 1) or 4714 Giulian and lngeman l CSFs as Promoters of Ameboid Microglia Figure 10. In vivo effects
of colony- stimulating factors upon adult brain. At 48 hr after injection of 10 units of m- multi-CSF (B), there was a significant increase in the
number of nonspecific esterase(+) cells compared with those sites receiving 50 units of rh-G-CSF (A) or PBS alone (C’). Scale bar, 20 pm. ( 3
t. I I. 100 ng but not in in be a but not 25 0 24 15 250 80 but not be an line in here, there , , 224: 375-387. Innocenti, G. M., S. Clarke, an
d H. Koppel (1983) Transitory mac- rophages in the white matter of the developing visual cortex. II. De- velopment and relations with axonal pathwa
ys. Dev. Brain Res. II: 55-66. Konigsmark, B. W., and R. L. Sidman (1963) Origin of brain mac- rophage in the mouse. J. Neuropathol. 22: 643-676. K
oski, I. R., D. G. Poplack, and R. M. Blaese (1976) A nonspecific esterase stain for the identification of monocytes and macrophages. In In C. D. E