PDF-Autoclave

SEB Training rev 101217 2 Autoclave Details 3 a utoclaves are located in SEB one on each upper floor Expensive pieces of equipment 70000 each Essential for several groups resea. Chamber Brite powder is simply the easiest way to keep autoclaves clean and free of water deposit buildup Designed specifically for steam sterilizers Chamber Brite effectively cleans and descales the reservoir chamber and tubing in minutes Cleaning Brite Cleaning Instructions:*1. Sprinkle Chamber Brite powder alongthe bottom of the COLD autoclavechamber.For best results, clean autoclave every 20cycles or once per week as needed. It isessential t Personal . Protective . Equipment. : What must be worn when you work in the laboratory.. 2. 1. . PPE. Eye Protection. Lab Coat. Long Pants. Closed Toed Shoes – no exposed skin around feet. Lab gloves – when required. In-charge. Sterilization. Disinfection & Sterilization. Disinfection : . The Destruction of all the Pathogenic organisms capable of giving rise to infection. Asepsis:. It is a technique to prevent infection by eliminating micro-organisms.. WWW - LDOW 2016, Canada. Nishita Jaykumar. , Pavankalyan . Yallamelli, . Vinh . Nguyen, . Sarasi Lalithsena, Krishnaprasad Thirunarayan, . Amit . Sheth . Kno.e.sis, Wright State Universit. y. Clare Paul. Individual labs must train new users on the safe usage of autoclaves.. Before use:. Check that autoclave is clear of any items left from previous use.. Check that door gaskets have not deteriorated. Loading. Used for sterilizing . machinary. . Autoclave Tape. Autoclave tape works by changing color after exposure to temperatures commonly used in sterilization processes, typically 121°C in a steam autoclave.. Historical Laboratory Research Safety. EHS Research Safety Checklist. Defined / limited focus . on chemical safety, FDNY / fire safety, general laboratory safety, hazardous materials shipment and emergency preparedness.. Most widely used method of sterilization by dry heat. . It is electrically heated and is fitted with a fan to ensure adequate and even distribution of hot air in the chamber. . It is also fitted with a thermostat which maintains the chamber air at a chosen temperature.. Sterilization concept . Sterilization is essential concept in the preparation of . sterile pharmaceutical products . .. . I. ts . aim is to provide a product that is . safe. and eliminates the possibility of introducing . What is this??. What is this?. What is this?. Aseptic Technique. Process of preparing and handling animals and equipment in such a way that the contamination of the patient through an open wound or incision is avoided. TITLE:Autoclave SterilizationSCOPE:Animal Care PersonnelRESPONSIBILITY:Facility Manager and Technical StaffPURPOSE:To Outline the Proper Procedures for Autoclave Sterilization PURPOSESterilization ref TABLE OF CONTENT1.0 2.0 3.0PERSONAL PROTECTIVE EQUIPMENT..................................4.05.0ITEMS FOR AUTOCLAVING ( THE CANS AND CAN NOTS )................6.0LOADING AN AUTOCLAVE................. II. Pipetting & . Reagent . Preparation. Winuthayanon Lab. Outline:. How to Use Pipette & Balance. Volumetric & Mass measurements. Glassware. Pipettes. Measurement . Balances. Pipetting & Reagent preparation.