PPT-Chapter 4 Gene expression

Acknowledgement Centers for Disease Control and Prevention Ethiopia CDCE American Society for Clinical Pathology ASCP Addiss Ababa University University of Gondar University of Hawassa. Inducible gene expression. kinetics of . β-galactosidase. enzyme induction. Add inducer. start transcription = mRNA accumulation. mRNA translation = protein accumulation. Remove inducer. Stop. transcription (. Bing Zhang. Department of Biomedical Informatics. Vanderbilt University. bing.zhang@vanderbilt.edu. Overall workflow of gene expression studies. Microarray. Biological question. Experimental . design. . in wounds undergoing negative pressure wound therapy. Nate . Stearrett. (presentation format shamelessly stolen from Jeff . Elhai. ). Chronic Wounds. http://wjes.biomedcentral.com/articles/10.1186/1749-7922-2-10. . Differential . expression, clustering, networks, and functional enrichment. STEMREM 201 Fall 2012. Aaron . Newman, Ph.D.. 10/17/12. A . genomics approach . to . biology involves…. A plethora of . University of Notre Dame. Kristin Hager, Associate Teaching Professor, Biological Sciences. Joseph O’Tousa, Professor, Biological Sciences. WH Freeman. Marc Mazzoni, Senior Editor, Life Sciences. Elaine Palucki, Editor, Adjunct Assistant Professor Biology, Brooklyn College. Draw 8 boxes on your paper. Gene regulation accounts for some of the phenotypic differences between organisms with similar genes.. 2005-2006. Gene regulation in bacteria. Control of gene expression enables individual bacteria to adjust their metabolism to environmental change. Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. Controlling gene expression is often accomplished by controlling transcription initiation.. Regulatory proteins . bind to DNA to either block or stimulate transcription, depending on how they interact with RNA polymerase.. Drosophila. Embryos using . lacZ. Transgenes. June 18. th. ABLE 2014. University of Oregon, Eugene. Cathy Silver Key. Julie Gates. Jessica Sawyer. Kirsten . Guss. Acknowledgements. Funding from . Roberta . Regulating . PROKARYOTIC. Gene Expression. Both prokaryotes and eukaryotes . alter their patterns of gene expression . in . response to changes in environmental conditions. .. During development, gene expression must be carefully regulated to ensure that the right genes are expressed only at the correct time and in the correct place.. We performed a genome-wide analysis of gene expressionto identify germline- and sex-regulated genes.Using mutants that cause defects in germ cell proliferationor gametogenesis, we identiÞed sets of g  which is designed to allow . expression.  (. transcription.  and . translation. ) of the inserted section of . DNA. . The . vector.  carries a . promoter.  (normally inducible) on one side of the . Pearley Chinta and Juliet V. Spencer . Abstract. Methods. Results. Conclusions. HCMV is a widespread pathogen in the general population and can cause severe disease in immune-compromised hosts. HCMV manipulates immune responses in several ways, one of which includes encoding genes with homology to host chemokine receptors. HCMV US27 encodes a chemokine-like receptor that stimulates host gene expression. While, no chemokine ligand has been identified for US27, it is constitutively active. US27 stimulates the gene expression of antioxidant response element (ARE) regulated genes by activation of the transcription factor nuclear respiratory factor 1 (NRF-1). The goal of this project is to identify specific host and viral genes that are regulated by US27. Increased expression of antioxidant genes is likely to benefit virus infection and enable more progeny virus to be produced. Thus, a better understanding of the US27 function has the potential to lead to the development of novel antiviral therapies necessary to treat HCMV infection. . Developing Ovule,. . Embryo and Endosperm. The 2. nd. . International . Conference on Transcriptomics. Jianbo. Wang. Wuhan University, China. Background. Materials and methods. Gene expression. .