PPT-Chapter 18 – Regulation of Gene Expression

Regulating PROKARYOTIC Gene Expression Both prokaryotes and eukaryotes alter their patterns of gene expression in response to changes in environmental conditions During development gene expression must be carefully regulated to ensure that the right genes are expressed only at the correct time and in the correct place. The problem to be solved (an example). Hauf, J., Zimmermann, F.K., M. ü. ller, S., 2000. Simultaneous genomic over expression of seven glycolytic enzymes in the yeast Saccharomyces cerevisiae. Ezyme. Microbiol. Technol.. of gene expression. . References:. . Stryer. : “Biochemistry”, 5. th. Ed.. Hames. & Hooper: “Instant Notes in Biochemistry”, 2. nd. Ed.. 1. Basic principles of gene control. What is gene expression?. Understanding:. Gene expression is regulated by proteins that bind to specific base sequences in DNA . The environment of a cell and of an organism has an impact on gene expression. Nucleosomes help to regulate transcription in eukaryotes. . Differential . expression, clustering, networks, and functional enrichment. STEMREM 201 Fall 2012. Aaron . Newman, Ph.D.. 10/17/12. A . genomics approach . to . biology involves…. A plethora of . r. esponses in the Pacific oyster, . Crassostrea. . gigas. . Mackenzie Gavery . University of Washington. School of Aquatic and Fishery Sciences. Committee Meeting: April 15. th. , 2014. Agenda:. Research update. Pre-transcriptional regulation. chromatin compaction . eg. . deacetylation. , . methylation. transcriptional initiation . ie. transcription factors to activate or repress. alternative promoters =. University of Notre Dame. Kristin Hager, Associate Teaching Professor, Biological Sciences. Joseph O’Tousa, Professor, Biological Sciences. WH Freeman. Marc Mazzoni, Senior Editor, Life Sciences. Elaine Palucki, Editor, Adjunct Assistant Professor Biology, Brooklyn College. THINK ABOUT IT . How Does A Cell Know?. Which Gene To . Express. &. Which Gene Should Stay . Silent. ?. Prokaryotic Gene Regulation. @. To conserve resources, prokaryotes regulate their activities, producing only those genes necessary for the cell to function.@ . Chapter . 16. Genes and Development. Proteins can determine the DNA sequence by binding the major groove of DNA.. Proteins binding the minor groove cannot determine the exact sequence of bases.. Prokaryotic gene regulation. CSCOPE. Unit: 08 Lesson: 01. There are hundreds of different types of cells in your body, and each type has a unique function. We’re going to compare some different types of cells to see how much they have in common. . Draw 8 boxes on your paper. Gene regulation accounts for some of the phenotypic differences between organisms with similar genes.. 2005-2006. Gene regulation in bacteria. Control of gene expression enables individual bacteria to adjust their metabolism to environmental change. . 1. The . trp. Operon. Contains 5 genes coding for proteins (enzymes) required for the synthesis of the amino acid tryptophan. Also contains a promoter and operator.. When tryptophan levels in the cell are low, the expression of the . Drosophila. Embryos using . lacZ. Transgenes. June 18. th. ABLE 2014. University of Oregon, Eugene. Cathy Silver Key. Julie Gates. Jessica Sawyer. Kirsten . Guss. Acknowledgements. Funding from . Roberta . Systematic . In . silico. . analysis using public database. Sung Hwan Lee. 1,4. , Baek Gil . Kim. 2,3. , . Ho Kyoung Hwang. 1,4. , Woo . Jung . Lee. 1,4. , Chang . Moo Kang. 1,4**.  . 1. Department .