PDF-Acid Stain - Flashconcrete

Need a Concrete Driveway Concrete Flooring or Concrete Repairs Flash Concrete are Christchurchs premium Concrete Contractor for all things Concrete. HANGING-DROP METHOD. USE OF SEMI-SOLID MEDIA. STAINING OF FLAGELLA. SEROLOGICAL TEST. FLUORESCENT TEST. SWARMING PHENOMENA. METHODS OF IDENTIFYNG MICROORGANISM. 2. MORPHOLOGY AND STAINING. 3 GENERAL WAYS. Objective: to understand how blood stains can give forensic scientists information about a crime.. blood. Blood is what moves oxygen around our body from the lungs to the organs that need oxygen and then back the lungs for more oxygen.. Cheng . Zhang. Thurs 1 Dec 11. MM Tutorial. Gram staining. Fixed film (heat kill bacteria etc...). Methyl violet . Lugol’s. /gram’s iodine. Decolourise with acetone. Methyl red .  . STAIN 2 (counter stain): pink-red colour. 2013-2014. بسم الله الرحمن الرحيم. Diagnostic Medical Microbiology-Laboratory Manual. An . etiological diagnosis of lower respiratory tract infection by microscopic examination and culture with identification and susceptibility test of . BIOLOGY & BIOTECHNOLODY DEPARTMENT. In our laboratory, . bacterial morphology . (form and structure) may be examined in two ways:. By observing . living unstained organisms. (. wet mount. ).. 2. By . A stain is a substance that adheres to a cell, giving the cell color. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . Bacterial Taxonomy: How are these unicellular organisms classified?. complex system of classification. based on shape & size; oxygen, pH, and temperature requirements; laboratory characteristics, biochemical . Gram stain . Ziehl. . Neelsen. stain. . Requires the use of at least 3 chemical reagents.. Primary Stain. : . stain all the cells.. Decolorizing agent: . may or may not remove the primary stain from the entire the cell or only from certain cell structure. . Basic vs. acidic dyes. Differential Stain. Primary stain (stains all cells on slide). Decolorizing step (removes stain from certain types of cells). Counterstain (stains the decolorized cells). COMPONENTS 100ml. MODIFIED MAYER . Almaarik. MYCOBACTERIUM. It has many species one of them is Mycobacterium tuberculosis that cause TB.. Some other common species seen include: . M. . avium.  AIDS patients. . M. . kansasii. M. . tissues, cells, cell components, cell contents, or microorganisms.. *The stain may be . natural or synthetic. . . *The object stained is called the . substrate. . . *Staining facilitates the observation of a substrate by introducing . & Mycobacterium. Bacterial shape. Bacilli. Cocci. Gram +ve. Gram –ve. Gram +ve. Neisseria spp.. Staphylococcus. Streptococcus. Gram -ve. Enterobacteriaceae. Spore forming:. 1. Bacillus spp. (aerobic). refractile. body within the vegetative cells. Procedure: (fig.10) . 1- Prepare a fixed smear.. 2- Flood the smear with 5% malachite green and let the stain react for 1 minute .Using your . bunsen. burner, heat the top of the stained slide until steam rises but do not let it boil. This step is performed by passing the burner back and over the slide for about 5 minutes..