PPT-Using BLAST for Genomic Sequence Annotation

Jeremy Buhler Adapted by Wilson Leung Last Update 08232022 Overview What is comparative annotation How to measure similarity between biosequences How to decide whether two sequences are similar enough. Basic Local Alignment Search Tool. (Altschul et al. 1990. ). What is blast. ?. BLAST .  DNA/protein databases. GOOGLE  internet. . D. Fristrom, Boston U. .. Uses for Blast. . Identifying unknown sequence. BCB 660. October 20, 2011. From Carson Holt. Annotations. Automated. Ab. initio. (based on genomic sequence alone). Involves comparisons to known proteins (BLAST similarity). Sequence motifs such as start/stop . Fikret Isik. Associate . Professor. September 16, 2013. Genomic BLUP - UofCopenhagen. 1. Cooperative Tree Improvement Program. North Carolina State University, Raleigh, . USA. Outline. Background. Realized genomic relationships. Drosophila. An introduction to web tools, databases and NCBI BLAST. Wilson Leung . 08/2016. Agenda. GEP annotation project overview. Web databases for . Drosophila. annotation. UCSC Genome Browser. Abstract. A large number of organizations today generate and share textual descriptions of their products, services, and actions. Such collections of textual data contain significant amount of structured information, which remains buried in the unstructured text. While information extraction algorithms facilitate the extraction of structured relations, they are often expensive and inaccurate, especially when operating on top of text that does not contain any instances of the targeted structured information. . et BLAST . avanc. é. J.. S. .. Bernardes. /H. . Richard. Mat. ériel. :. Bioinformatics and Functional Genomics. Jonathan Pevsner, . Wiley-Blackwell. ed.. Outline. Introduction. . Definition of . 03/14/2017. 1. Leonardo . Mariño-Ramírez. NCBI, NIH – Bethesda, USA. NCBI Search Services and Tools. Entrez. . integrated literature and molecular databases. Viewers. BLink. . protein similarities. . Jundi. Wang. School of Computing. CSC691. 09/08/2013. 2. Overview. 1.Introduction of BLAST . Background of BLAST. Programs in BLAST. Function of BLAST. 2.Application of BLAST. Chris Shaffer July 2012. Last update: 08/2018. Annotation . “Big Picture” of annotation and then one practical example. This technique may not be the best with other projects (e.g. corn, bacteria). Concepts. Introduction to Biostatistics and Bioinformatics. Sequence . Database Searching. This Lecture. Stuart M. Brown, Ph.D.. Center for Health Informatics and Bioinformatics. NYU School of Medicine. BLAST. Local pairwise alignment heuristic. Faster than standard pairwise alignment programs such as SSEARCH, but less sensitive.. Online server: . http://www.ncbi.nlm.nih.gov/blast. BLAST. Given a query . Last Updated: 12/26/2021. Wilson Leung and Chris Shaffer. Agenda. Overview of the GEP annotation project. GEP annotation strategy. Types of evidence. Analysis tools. Web databases. Annotation of a single isoform (walkthrough). Dina Paltoo, Ph.D., M.P.H.. NIH Office of Science Policy. Laura Lyman Rodriguez, Ph.D.. National Human Genome Research Institute. October 4, 2017. Housekeeping. Participants must register to join the webinar. online and offline. Wen-Dar Lin. Bioinformatics core, IPMB. wdlin@gate.sinica.edu.tw. Preface. When we are talking about . sequence similarity. , we usually are talking about . local alignments. .. NCBI BLAST is one of the most famous local alignment programs where almost everyone has ever used it..