PPT-How To Blast David A. Kloske

Thermo Fisher Open Biosystems Dr Krishnan K Chittur University of Alabama in Huntsville Dr Joseph Ng University of Alabama in Huntsville What is BLAST BLAST stands for B asic L. Yanbin Yin. Fall 2014. 1. http://. www.ncbi.nlm.nih.gov. /books/NBK52640/. 2. Why you do need to run BLAST in command line terminal?. NCBI’s server does not have a database that you want to search. Mohammed Mehdi Rizvi. Molecular Lab Techniques 446. Background. BLAST is the . B. asic . L. ocal . A. lignment . S. earch . T. ool. . It is an algorithm used for comparing sequences, such as amino acid sequences of proteins, or the nucleotides of DNA sequences.. Guidance on post-blast fumes and what steps to take to avoid exposure to them. . Drilling and Blasting. Toolbox Talk : 16. Target Audience. All those involved in the blasting operation – especially the post-blast inspection process.. et BLAST . avanc. é. J.. S. .. Bernardes. /H. . Richard. Mat. ériel. :. Bioinformatics and Functional Genomics. Jonathan Pevsner, . Wiley-Blackwell. ed.. Outline. Introduction. . Definition of . 03/14/2017. 1. Leonardo . Mariño-Ramírez. NCBI, NIH – Bethesda, USA. NCBI Search Services and Tools. Entrez. . integrated literature and molecular databases. Viewers. BLink. . protein similarities. Recap : . Pairwise. sequence alignment. Grouping of two sequences to maximize similarity/identity using a scoring system (substitution matrices):. PAM (1,120,250). BLOSUM (80,62,45). Optimal alignment algorithms:. Xuhua Xia. xxia@uottawa.ca. http://dambe.bio.uottawa.ca. Why string matching?. Early applications: . Sequence similarity between an oncogene (genes in viruses that cause a cancer-like transformation of the infected cells), v-sis, and the platelet-derived growth factor (PDGF). Adapted from College Board’s “Investigation 3: Comparing DNA Sequences to Understand Evolutionary Relationships with BLAST”. What we know so far.... We already know that DNA can be used to identify animal species at a genetic level. 1 Samuel 16-31. The preserving grace of God. 2. David, the triumphant king. 2 Samuel 1-10. God’s enriching grace. 3. David, the troubled king. 2 Samuel 11 to 1 Kings 2. God’s over-coming, forgiving grace. Outline. A brief introduction on various kind of BLAST. Different Sequences: introduction of NCBI and FASTA format. Web version BLAST. BLAST on Linux system. An application of BLAST on Bioengineering. Outline I ntroduction BLAST s earch s teps Step 1: Specifying Sequence of interest Step 2: Selecting BLAST Program Step 3: Selecting a Database Step 4: Selecting Search Parameters and Format Team2. 邱冠儒 黃. 尹. 柔 田. 耕. 豪 蕭. 逸. 嫻 謝朝茂 . 莊閔. 傑. 2014/05/12. 1. BLAST. Basic local alignment search tool. Enable to compare query sequence of amino-acid or protein or DNA to database . Construction Trades Training (9 Weeks). Learn the Theory and technical aspects of Construction . . . . Computer Science IT Bootcamp (7 Weeks). Learn Computer Science and Information Technology (IT). Arya L, Rathinam SR, Lalitha P, Kim UR, Ghatani S, Tandon V. Trematode Fluke Procerovum varium as Cause of Ocular Inflammation in Children, South India. Emerg Infect Dis. 2016;22(2):192-200. https://doi.org/10.3201/eid2202.150051.