PPT-Evaluation of solid-phase chromatography as a method for natural organic matter characterization

Dr Aleksandra Tubić Dr Jasmina Agbaba Dr Jelena Molnar Jazić Dr Božo Dalmacija Depart ment of Chemistry Biochemistry and Environmental Protection Faculty of Science University of Novi . Commercial. Institutional. Construction and demolition. Municipal services. Treatment plant sites. Industrial. Agricultural. Biomedical waste. Municipal solid waste (MSW) . Industrial solid waste . Hospital waste. Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography HPLC- High Performance Liquid Chromatography IR – Infrared Spectroscopy UV-Vis Spectroscopy MS – Mass Spectrometry BASICS OF CHROMATOGRAPHY Prepared by DR: Sherif Abdel- Naby Abdel- Gawad PhD Analytical Chemistry-Faculty of Pharmacy - Cairo University History of Chromatography Mikhail Tswett is credited with GC . is currently one of the most popular methods for separating and analyzing compounds. . GC can be applied to the separation of any compound that is either naturally volatile (i.e., readily goes into the gas phase) or can be converted to a volatile derivative.. ASSISTANT PROFESSOR. PG&RESEARCH DEPARTMENT OF BIOTECHNOLOGY. BONSECOURS COLLEGE FOR WOMEN. THANJAVUR. WHAT IS THE . DOWNSTREAM . PROCESSING. Downstream processing refers to the recovery and purification of biosynthetic products, particularly pharmaceuticals, from natural sources such as animal or plant tissue or fermentation broth, including the recycling of salvageable components and the proper treatment and disposal of waste. It is an essential step in the manufacture of pharmaceuticals such as antibiotics, hormones (e.g. insulin and humans growth hormone), antibodies (e.g. infliximab . 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University. INTRODUCTION: GAS CHROMATOGRAPHY . Origin of gas chromatography: 1905, W. Ramsey . Separated mixture of gases and vapors . Used solid adsorbent: activated charcoal. . A sample containing the materials to be separated is injected into the gas chromatograph. . Gas Chromatography. A mobile phase (carrier gas) moves through a column that contains a wall coated or granular solid coated stationary phase. . Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . By . Dr. . Amjad. M. T. Khan. Assistant Professor. Department of Chemistry. AMU, Aligarh. CONTENTS. Introduction to chromatography. History. Principles. Importance. Chromatographic terms. Classification of chromatography. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. Carotenoids. . T.A Nouf Alshareef. KAU-Faculty of Science- Biochemistry department . Analytical biochemistry lab (. Bioc. 343) 2012. nf.shareef@hotmil.com. . Background . Carotenoids. are organic, fat-soluble pigments that . Separation Technique, . Chromatography.  . For Postgraduate (. Master's degrees).  . Lecture No. 1.  By . Asst. Prof. Dr. Layla Salih Al-Omran . Chromatography.  . Background . Chromatography is a technique in which substance are separated, purified and identified from a mixture for qualitative and quantitative analysis. . . . CHROMATOGRAPHIC TECHNIQUES. CONTENTS. Introduction to chromatography. History. Principles. Importance. Chromatographic terms. Classification of chromatography. Adsorption chromatography. Partition chromatography. Introduction, theory, instrumentation, . derivatization. ,. Temperature programming, advantages, disadvantages and applications. 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University.