PPT-Week 4: Functional Genomics via Mass Spectrometry

April 15 Bafna Peptide MS Instrument software usually detects peaks and computes features peak area mz April 15 Bafna mz Nobel Citation 2002 April 15 Bafna Nobel Citation 2002 April 15. Keywords. :. Molecular . ion (M. +). Fragmentation. Starter:. Use mini wipe boards to brainstorm what you know about mass spectrometry. A: . Use . the molecular ion peak in an organic . molecule’s . PUMP How the magnetic sector mass spectrograph works: qvB = mv), B the magnetic field, m is the mass of the Classically, the speed of the ion, v, is related Courtesy www.lab-initio.com. Purpose of Mass Spectrometry. P. roduces.  . spectra of masses from . the molecules . in . a sample of . material, and fragments of the molecules.. U. sed . to determine . Objectives of the presentation. To get an appreciation of the role of . physics . in . our day to day living. Acquaint ourselves to a novel and . widely used . research tool: Mass Spectrometer. To . introduce the basic physics of mass . Jonathan A. Karty, Ph.D.. jkarty@indiana.edu. http://msf.chem.indiana.edu . Why Mass Spectrometry. Information is composition-specific. Very selective analytical technique. Most other . spectroscopies. Objectives of the presentation. To get an appreciation of the role of . physics . in . our day to day living. Acquaint ourselves to a novel and . widely used . research tool: Mass Spectrometer. To . introduce the basic physics of mass . Fenyő. Readings for Today. http://fenyolab.org/pi2019. Ion Source. Mass Analyzer. Detector. mass/charge. intensity. Mass spectrometry . Mass Analyzer 1. Frag-mentation. Detector. Ion Source. Mass Analyzer 2. Lecture 1. 4/13/2017. Announcements. Homework 2.2 Additional Problems - Due. Quiz Today (after Announcements). 4/18 Lecture – will go back to rest of NMR (interpretation examples and instrumentation), then continue on Mass . &. Secondary. . ion. . mass. . spectrometry. (SIMS). L. Seda Mut 20970802 . Neslihan . Ötük. 20622809. Beytepe. Ankara. 12.04.2012. Outline. Historical Background of MS. . and. SIMS. What is M. and Andrew Gioe. Computation of Molecular Mass. Mass Spectrum is a plot of intensity as a function of mass-charge ratio, m/z. . Mass-charge ratio can be determined through accelerating an ion.. Conti. Mass Spectrometry deals with the examination of characteristics fragments and arising from the breakdown of organic molecules. The mass spectrum is the plot of relative intensity of the ions agains SOP template 1 Revised: 06 /2019 MTD Standard Operating Procedure (SOP) This Standard Operating Procedure (SOP) describes basic chemical safety information for Mass Spectrometry (MS) . Prior to con Outline : Functional genomicsIntroductionRelation between genotype and phenotypeEight model organismsE. coli; yeast; ArabidopsisC. elegansDrosophilazebrafish; mouse; humanFunctional genomics using re What . is . Functional. . Genomics?. RNA . Transcription/Gene. . Expression. Measuring Gene. . Expression. Microarrays. High-throughput. . Sequencing. Transcriptional. . Regulation. Transcription.